FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765691
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25790.3368199443378595No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25390.3315959048754654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19300.25205990406051526No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17360.22672331266790388No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16230.2119654011866406No Hit
GGCTAACAGTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT15770.20595775580488734No Hit
GTTATAACCCCTCCCTCAAGAGTCGAGTCACCATATCGATAGACACGTCC15330.20021131239625384No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCCGTC14560.1901550364311452No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTCCCA14160.1849309969687511No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14010.18297198217035332No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13930.1819271742778745No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13760.179706957506357No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13630.17800914468107892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13480.17605012988268115No Hit
GTCTCTGGTGACTCCGTCAGCAGTGAAACTTACTACTGGACCTGGATCCG12950.16912827759500895No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12400.16194522333421707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12240.15985560754925943No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11960.15619877992558354No Hit
GTTGGAGACAGAGTCACTATCACTTGTCGGGCGAGTCAGGATATTCACACCTGGTTAGCCTGGTATCAGCAGAAA11700.1528031542750274No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGGTCGGTC11400.1488851246782318No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11380.1486239227051121No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10990.14353048422927786No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10850.14170207041743993No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10720.14000425759216187No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTG10480.1368698339147254No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10410.13595562700880642No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTCCCACATGCGGCGC10290.1343884151700882No Hit
GATTTCACTCTCACCATCAGTAGCCTGCAGGCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC10260.13399661221040862No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCCTCAGGGA10020.13086218853297216No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9990.13047038557329263No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGAATGGGAAA9970.13020918360017292No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCCTCAGGGAGA9640.1258993510436978No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9630.12576875005713792No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9310.12158951848722264No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9120.11910809974258546No Hit
GCCCCTAACCTCTTAATCTATGGTGCATCCATTTTGCAAACTGGGGTCCC9040.11806329185010662No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8890.11610427705170884No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC8760.11440646422643076No Hit
GAGTGAAATCCGTCCCATATCCACTGCCGCTGAACCTTGATGGGACCCCA8600.11231684844147313No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8570.11192504548179358No Hit
TCAGTAGCCTGCAGGCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCATTCACTTTCGGCC8450.11035783364307535No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8370.10931302575059652No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8260.10787641489843813No Hit
GGTTAGGGGCAGTCCCTGGTTTCTGCTGATACCAGGCTAACCAGGTGTGA8210.10722340996563888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8040.1050031931941214No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8030.10487259220756154No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7850.1025217744494842No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7840.10239117346292434No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7840.10239117346292434No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7810.10199937050324479No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7790.10173816853012507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTT150.002253421169.7731448
AATCGTC200.00774403551.08513315
GTGGTAT1800.041.6494181
GTATCAA18050.040.967671
AGGTAAT350.001149906140.7208260
TATCAAC20300.036.3864752
ATCAACG21250.034.755243
AACGCAG21400.034.357086
TCAACGC21400.034.3525924
CAACGCA21650.033.958135
CGCAGAG22050.033.653038
GCAGAGT22700.032.6893969
CAGAGTA22500.032.5261210
AGAGTAC22450.032.44693811
ACGCAGA23100.031.9759947
GAGTTTA2200.030.9789051
AGTACTT5750.030.1950213
TGTTAGA7150.029.0141370
AGTTTAT2500.028.5927262
AGCCGTG605.707091E-428.4979531
Produced by FastQC (version 0.11.5)