FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549652
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68631.2486082102857807No Hit
GATCTATACTGCATCCAGTTTGCAAAGTGGGGTCCCACCCAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA18880.3434900628033738No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18120.32966313230917016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16010.2912752068581575No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12400.22559728701069037No Hit
GTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11910.2166825555078486No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10360.1884828946315123No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10070.18320682904819777No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9150.16646896581837234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9020.16410383297067963No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8970.16319416649079782No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8660.15755423431553056No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAGA8620.1568265011316251No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8390.15264203532416876No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8270.1504588357724524No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8070.14682016985292512No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7720.1404525044937524No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7630.13881510482996515No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7550.13735963846215424No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7550.13735963846215424No Hit
GTGTATTACTGTGCGGAGGTATTACGATATTTTGACTGGGTAGGAGGTAAGAACTACCACTACTACATCGACGT7550.13735963846215424No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7510.13663190527824878No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTCCAGTACC7380.13426677243055607No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7350.13372097254262696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7320.1331751726546979No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7230.13153777299091063No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCGATTTAAATTGGTATCAGCAGAAA7220.13135583969493425No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6960.1266255739995488No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG6710.12207724160013973No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6650.12098564182428154No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6630.12062177523232882No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6420.1168011760168252No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6240.11352637668925065No Hit
ATACTGCATCCAGTTTGCAAAGTGGGGTCCCACCCAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCA6230.11334444339327429No Hit
ACTTACTACTGTCAACAGAGTTCCAGTACCCTCACGTGGACGTTCGGCCA6170.11225284361741611No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6020.10952384417777065No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6000.10915997758581793No Hit
GTTCCAGTACCCTCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6000.10915997758581793No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5980.10879611099386521No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5950.10825031110593612No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC5820.1058851782582434No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA5820.1058851782582434No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT5810.10570324496226703No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC5750.10461164518640886No Hit
GTACTGGAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATT5560.10115491256285797No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5530.10060911267492885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGAT2600.049.38468670
GTGGTAT2050.045.58811
GTATCAA19800.034.2198561
GCTTGTA502.2592804E-433.3770071
GCAATGG400.003071344733.3344886
CCCGTCG450.00401823531.55434849
TATACTA450.005466153429.6306515
AATCGCT3550.029.3458153
TATCAAC22950.029.1922512
CAGTTCG7250.028.89586670
ACGGGAC704.8365087E-528.59583316
CGTGTCA2650.028.39264164
AAATCGC3550.028.2535452
ATCAACG23750.028.2038023
GAGAACT2800.027.69055247
TCAACGC23950.027.6899874
ATCGCTG3650.027.65523754
TTAGACG2800.027.54481359
GCTTGAA855.7208927E-627.4869441
CAACGCA24150.027.4681685
Produced by FastQC (version 0.11.5)