FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331838
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTCTTGGAGCTGTATAAAAGACT54840.41176179084843656No Hit
CTTTTATACAGCTCCAAGAATAAGAACTACTTAGCTTGGTACCAGCAGAA54720.41086078036517953No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48960.36761227716884487No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT44690.33555132080628425No Hit
ATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT42910.3221863319713058No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41620.3125004692762934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40610.30491696437554716No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACAGAGCCAGGGACTCTGGAGA37370.2805896813276089No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33650.2526583563466428No Hit
CTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC33560.2519825984842No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33440.2510815880009431No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30100.22600346288362397No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAAGAATAAGAACTA30040.22555295764199548No Hit
GTACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT28890.2169182738441162No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28330.21271355825558366No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28200.21173746356538858No Hit
TTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT26580.19957382204141946No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25980.19506876962513459No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25870.1942428433488157No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25110.18853644362152153No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24810.1862839174133791No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23560.17689839154611897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23300.17494620216572887No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22240.16698727623029228No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21900.1644344131943975No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21810.16375865533195477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21390.16060511864055538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21310.16000444498505073No Hit
GTCTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA21110.15850276084628911No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19540.1467145403570104No Hit
GTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCGGATTCCCGGGT18820.14130847745746855No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCG18640.1399569617325831No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGCC18350.13777951973137875No Hit
ACCTTGGTCCCTCCGCCGAAAGCCGGAGGAGTACTATAATATTGCTGACAGAAATAAACTGCCACATCTTCAGC18310.1374791829036264No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18090.13582733035098865No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACGCAGTCTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGG17260.1295953411751279No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAATATTATAGTACT16950.1272677307600474No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCTACCCGGGAA16750.12576604662128577No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.1256158782074096No Hit
CTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGC16280.12223708889519595No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16240.12193675206744363No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16230.12186166786050556No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC15980.11998456268705353No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15790.11855796275522998No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15530.11660577337483989No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15440.11593001551239715No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15400.11562967868464483No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15390.11555459447770676No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACC15270.11465358399444978No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15250.11450341558057363No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14870.11165021571692653No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14740.11067412102673146No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTCTTGGAGCTG14530.10909735268103177No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG14450.10849667902552713No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13870.10414179502311843No Hit
GTTTATTTCTGTCAGCAATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAA13700.10286536350517105No Hit
GTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA13480.10121351095253328No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA13440.10091317412478093No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA13370.10038758467621436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTTA200.00551937855.6540766
GGTATCA11600.043.481571
GTATCAA29550.040.0947461
GTGGTAT3350.038.398631
ATCAACG35500.033.1540263
TGGTATC4000.032.9778752
CAACGCA36050.032.651895
TCAACGC36000.032.599614
AACGCAG36550.032.4852876
TATCAAC36350.032.4742432
ACGCAGA37950.031.0194727
CGCAGAG38500.030.5763368
GCTTGTG1001.7445927E-830.4662781
AGAGTAC38650.030.19510511
CAGAGTA39100.030.10713610
TTAACGG1600.029.90088735
TCTAGGG900.005298348629.82120970
GCAGAGT39800.029.5776129
TGTTAGA15150.029.23069270
GTGCTCA27750.028.04805870
Produced by FastQC (version 0.11.5)