FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094771
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTATTCCATTCACTTTCGGCCCTGGGACCAAAGTGAATATCAAACGAACTGTGGCTGCACCAT43080.3935069525955657No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41460.37870933738654017No Hit
GTGGTAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA39290.35888784047074684No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38920.35550813823164845No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTGGTTCTTATAGTTGGAGGTGTAGAAAACACT28780.2628860282196002No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28040.25612662374140344No Hit
CTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27940.2552131907038093No Hit
CTCCTAAACTACTCATTTACGAGGCATCTACGCGGGAGTCCGGGGTCCCC27240.24881915944065014No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26470.2417857250511751No Hit
GTCTACCACTGTGCGAGAGATGCGGCGGAACCGGGGGCTCGACTGGGCGTTATTGAAACCTGGGGCCAGGGAACC24660.22525258707072074No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24020.2194066156301181No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23730.216757659821095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23660.2161182566947791No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23130.21127706159553No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23120.2111857182917706No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23020.21027228525417646No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22990.2099982553428982No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21440.19584004326018864No Hit
GTGTTGCAGACCCAGGTCTTCATTTTTCTGTTGCTCTGGATCTCTGTGAC20100.18360004055642687No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT19070.17419168026920698No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18740.17117735124514624No Hit
GTTTATTACTGTCAACAATATTATAGTATTCCATTCACTTTCGGCCCTGGGACCAAAGTGAATATCAAACGAA18020.16460063337446826No Hit
ATAGTATTCCATTCACTTTCGGCCCTGGGACCAAAGTGAATATCAAACGA17840.16295645390679878No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17620.1609469012240916No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17560.1603988414015351No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17530.16012481149025687No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17470.15957675166770036No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17330.15829794541506853No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACGAGGCATCTACGCGGGAG16990.1551922730872484No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16810.15354809361957888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16370.14952898825416458No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15850.1447791364586749No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTGGTTCTTATAGTTGGAGGTG15470.1413080909158171No Hit
CTATAAGAACCACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTA15200.13884182171431286No Hit
GTTTTCTACACCTCCAACTATAAGAACCACTTAGCTTGGTTCCAGCAGAA15080.13774570206919987No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15020.13719764224664335No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14710.13436599983010147No Hit
GGATCTCTGTGACTACAGGTGCCTACGGGGACATCGTGATGACCCAGTCG14580.13317853688122905No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTTTCTGTT14330.13089495428724363No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14330.13089495428724363No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14190.1296161480346118No Hit
GTCCCCGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14170.12943346142709297No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14120.1289767449082959No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14110.1288854016045365No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTATT13740.12550569936543807No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13700.1251403261504004No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13670.12486629623912215No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGGAAGCCAGAGGTAC13650.12468360963160331No Hit
CTGTGACTACAGGTGCCTACGGGGACATCGTGATGACCCAGTCGCCAGAC13500.12331346007521207No Hit
GTGCAGCCACAGTTCGTTTGATATTCACTTTGGTCCCAGGGCCGAAAGTG13210.120664504266189No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13180.12039047435491075No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13150.1201164444436325No Hit
GTCGGGGACCCCGGACTCCCGCGTAGATGCCTCGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGAA12970.118472264975963No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12820.11710211541957176No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12580.11490987612934576No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTCTACACCTCCAACTATAAGAACCA12570.11481853282558636No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12520.11436181630678927No Hit
CTCCCTGGCTTTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTCTACACCTCCAA12430.11353972657295454No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12040.10997733772633729No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11950.10915524799250255No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11940.10906390468874312No Hit
CTCCTGTGCAACGTCTGGATTCACCTTCAGTACCTCTGGCTTCCACTGGGTCCGCCAGGCTCCAGGCAAGGGG11830.10805912834738955No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11640.10632360557596063No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC11600.10595823236092299No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11600.10595823236092299No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11600.10595823236092299No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11560.1055928591458853No Hit
ATATATGTTCCTTCCATCATACCAAATCATTGCCACCCACTCCAGCCCCT11540.10541017253836649No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC11470.10477076941205056No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11420.10431405289325349No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11400.10413136628573463No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11390.10404002298197523No Hit
GGGCTGGAGTGGGTGGCAATGATTTGGTATGATGGAAGGAACATATATTA11380.1039486796782158No Hit
GAACATATATTATGCAGACTCTGTGAACGGCCGATTCACCATCTCCAGAGACAATTACAAGAACACACTGTCT11320.1034006198556593No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11290.10312658994438106No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT11220.10248718681806514No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11080.10120838056543331No Hit
CTTTCGGCCCTGGGACCAAAGTGAATATCAAACGAACTGTGGCTGCACCA11050.10093435065415507No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11050.10093435065415507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)