FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094771
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTATTCCATTCACTTTCGGCCCTGGGACCAAAGTGAATATCAAACGAACTGTGGCTGCACCAT45640.41689083835797625No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39590.3616281395835293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38400.3507582864361588No Hit
GTGGTAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAGACA37460.3421720158827737No Hit
CTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC35530.3245427582572063No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34950.31924484663916014No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29850.2726597617218578No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTGGTTCTTATAGTTGGAGGTGTAGAAAACACT27310.24945856256696608No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25730.23502632057297826No Hit
GTCTACCACTGTGCGAGAGATGCGGCGGAACCGGGGGCTCGACTGGGCGTTATTGAAACCTGGGGCCAGGGAACC24570.22443049733688597No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24220.22123348170530638No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23000.2100895986466576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22690.20725795623011573No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22590.20634452319252153No Hit
CTCCTAAACTACTCATTTACGAGGCATCTACGCGGGAGTCCGGGGTCCCC22210.20287347764966374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22050.20141198478951303No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21920.20022452184064063No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21160.19328243075492502No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19110.17455705348424466No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18840.1720907842827404No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18510.16907645525867965No Hit
GTGTTGCAGACCCAGGTCTTCATTTTTCTGTTGCTCTGGATCTCTGTGAC18210.16633615614589717No Hit
GTTTATTACTGTCAACAATATTATAGTATTCCATTCACTTTCGGCCCTGGGACCAAAGTGAATATCAAACGAA17690.16158630435040752No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17620.1609469012240916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17550.1603074980977757No Hit
ATAGTATTCCATTCACTTTCGGCCCTGGGACCAAAGTGAATATCAAACGA17420.15912003514890327No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCGGGGACCCCGGACTCCCGCGT17050.15574033290980488No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.14962033155792398No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16210.1480674953940139No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16130.14733674896393859No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15750.14386570342108074No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15690.14331764359852428No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCGGGGACCCCG15180.13865913510679403No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTGGTTCTTATAGTTGGAGGTG14710.13436599983010147No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14430.1318083873248378No Hit
GTGCAGCCACAGTTCGTTTGATATTCACTTTGGTCCCAGGGCCGAAAGTG14000.1278806252631829No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGGAAGCCAGAGGTAC13750.1255970426691975No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13380.12221734043009908No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13320.12166928060754258No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCGGGGACC13310.12157793730378316No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATAGTATT13180.12039047435491075No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13140.1200251011398731No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACGAGGCATCTACGCGGGAG13110.11975107122859485No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13060.11929435470979775No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA12930.11810689176092536No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12890.11774151854588769No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12660.1156406225594211No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12610.115183906040624No Hit
CTCCTGTGCAACGTCTGGATTCACCTTCAGTACCTCTGGCTTCCACTGGGTCCGCCAGGCTCCAGGCAAGGGG12600.1150925627368646No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12580.11490987612934576No Hit
GTCGGGGACCCCGGACTCCCGCGTAGATGCCTCGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGAA12570.11481853282558636No Hit
GGATCTCTGTGACTACAGGTGCCTACGGGGACATCGTGATGACCCAGTCG12400.11326569666167628No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTTTCTGTT12390.11317435335791687No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12330.11262629353536037No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12320.11253495023160094No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12150.11098211406769087No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12040.10997733772633729No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12000.10961196451129962No Hit
GAACATATATTATGCAGACTCTGTGAACGGCCGATTCACCATCTCCAGAGACAATTACAAGAACACACTGTCT11900.10869853147370545No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11900.10869853147370545No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11830.10805912834738955No Hit
GTTTTCTACACCTCCAACTATAAGAACCACTTAGCTTGGTTCCAGCAGAA11640.10632360557596063No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11600.10595823236092299No Hit
GTCCCCGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC11480.10486211271580999No Hit
CTGTGACTACAGGTGCCTACGGGGACATCGTGATGACCCAGTCGCCAGAC11450.10458808280453173No Hit
CTATAAGAACCACTTAGCTTGGTTCCAGCAGAAACCAGGACAGCCTCCTA11330.10349196315941873No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC11250.1027612167293434No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11220.10248718681806514No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11180.1021218136030275No Hit
ATATATGTTCCTTCCATCATACCAAATCATTGCCACCCACTCCAGCCCCT11120.101573753780471No Hit
GAATACTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT11080.10120838056543331No Hit
GGGCTGGAGTGGGTGGCAATGATTTGGTATGATGGAAGGAACATATATTA10980.10029494752783914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC200.00786921350.87980315
GTGGTAT2250.049.767331
GGTACCG306.876791E-445.2058266
TCTGGAG12650.043.86989270
TGGTATC2700.041.4386752
GACAGGT1650.041.1300241
GGTATCA7550.040.4490661
GTATCAA19550.039.0524861
GGTAGAC8900.036.1884043
TCGGCTC8950.036.02902220
CAGCCGT8750.035.6480269
TAGACAG9050.035.586975
AGCCGTG8900.035.4281610
CGGCTCT9050.035.2606921
GCCGTGT9150.034.0896411
ACAGCCG9250.034.0876358
CGTTAAA502.0552613E-433.92296617
GTACCGC502.061892E-433.9043737
TATCAAC23050.033.2424622
TGGTAGA9900.032.5344962
Produced by FastQC (version 0.11.5)