FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305584
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52161.7068956489868579No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11930.3904000209435049No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10260.335750562856694No Hit
GAGTATTAGTCCCTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGGTCCTGATCTATAGGGCGT8890.29091837268966964No Hit
GATCTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCA8580.28077386250589037No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7810.2555762081784387No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7350.2405230640347662No Hit
GTATGACAGTTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT7090.23201476517095135No Hit
CTGTCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC6400.2094350489554427No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6250.20452641499554952No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6160.2015812346196136No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC5910.19340017801979162No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5770.1888187863238913No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5690.18620084821194827No Hit
GTATTAGTCCCTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCT5530.18096497198806222No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5480.1793287606680978No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5220.17082046180428295No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4990.16329388973244674No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4890.16002146709251794No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTCCCTGGTTGGCCTGGTATCAGCAGAGA4870.15936698256453216No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG4780.15642180218859628No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4710.1541311063406461No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4690.15347662181266036No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4660.1524948950206817No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA4600.15053144143672442No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4520.1479135033247814No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4510.14758626106078854No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4500.14725901879679565No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4250.13907796219697366No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGACAGTTAT4230.1384234776689879No Hit
CTATAGATCAGGACCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC4190.13711450861301638No Hit
CAGTATGACAGTTATCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT4160.13613278182103775No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4140.135478297293052No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT4130.1351510550290591No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4120.13482381276506622No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4080.13351484370909472No Hit
GTCCTGATCTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT4040.13220587465312322No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4020.13155139012513745No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG4020.13155139012513745No Hit
GTCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC3920.12827896748520864No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3850.12598827163725848No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGATAA3800.1243520603172941No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG3680.12042515314937956No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA3580.11715273050945076No Hit
GACCAGGGAAAGCCCCTAAGGTCCTGATCTATAGGGCGTCTAGTTTAGAA3560.116498245981465No Hit
GAGTGAATTCTGTCCCAGATTCACTGCCGCTGAACCTTGATGGGACCCCA3490.11420755013351486No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT3410.11158961202157182No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3400.11126236975757893No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAGGTCCTGATCTATAGGGCGTCTAGTTTAGAAAGTGGGGTCCCAT3380.11060788522959317No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3360.10995340070160742No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC3350.10962615843761454No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3220.10537200900570712No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT3170.1037357976857427No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT3160.10340855542174981No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTCC3130.10242682862977119No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC3120.10209958636577832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTT150.002760622966.281015
TTGATCG200.00861493349.71889510
CAATCTT1007.1413524E-848.08071570
ATATGTC504.0480063E-641.0727237
GTGGTAT1800.040.524961
CATTAGA458.795323E-539.17590346
CCATTAG458.995753E-539.026445
GGAAAAC501.0410712E-438.068965
TATGTCT557.6096185E-637.46549238
ATAGGTC451.2152047E-437.08376726
TATATGC451.2655725E-436.8288113
GTGTAGA755.6794306E-835.367241
TCAATTA502.1609385E-433.6247931
TAGGTCA502.2422915E-433.41394427
ACATAGG502.2971802E-433.2766624
GCATAAA502.3239182E-433.21116618
ATATGCA502.3509128E-433.1459314
GTATCAA9100.032.063711
CATAGGT553.9714956E-430.31128725
CATAAAC554.0604704E-430.19692619
Produced by FastQC (version 0.11.5)