FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783706
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24260.3095548585821724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22010.28084511283568075No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA20420.26055689250815994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17300.22074604507302484No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17260.22023564959308717No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAATAGCAACTACATAGCCTGGTACC16620.21206932191408512No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTGATTTGCAGATATA16420.209517344514397No Hit
TATCAATACTGATGGGGGTAGTGCGACGTACGCGGACTCCGTGGAGGGCCGATTCACCGTCTCCAGAGACAACGC16310.2081137569445685No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15200.19395028237629927No Hit
GTGTTAATAGCAACTACATAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACAT14590.1861667513072504No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13860.17685203379838868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13350.1703444914291839No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13330.1700892936892151No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCTT13190.16830290950943338No Hit
GTGTATTACTGTACAAGAGGGGGTTGGTACATGGACGTCTGGGGCAAGGG12670.1616677682702442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12650.16141257053027538No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12520.1597537852204781No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGAGTGTTAATAG12260.1564362146008835No Hit
CTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGC12100.154394632681133No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12080.15413943494116414No Hit
GTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGA12060.15388423720119535No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11990.15299104511130449No Hit
CTATTATTGTCAATACTATTATAACTCTCAATTCACTTTCGGCCCTGGGA11780.15031146884163193No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11610.148142288051897No Hit
GTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATCCCA11590.1478870903119282No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11530.14712149709202174No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT11530.14712149709202174No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTGGACTGGAGCCTGAAGATT11520.14699389822203734No Hit
CCCTATATCTGCAAATCAACAGTCTGAGAGCCGAGGACACGGCTGTGTAT11220.1431659321225051No Hit
CTATTATAACTCTCAATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT10980.14010355924287934No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10950.13972076263292613No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10890.1389551694130197No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10730.13691358749326918No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10640.1357651976634095No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10520.1342340112235966No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10390.1325752259137993No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10330.13180963269389284No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10300.13142683608393965No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC10290.13129923721395523No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10180.12989564964412675No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10130.12925765529420472No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10110.1290024575542359No Hit
GTATTGATACGTGTGATCCACTCCAGCCCCTTCCCCGGAGCTTGGCGGAC9970.1272160733744542No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9790.12491929371473486No Hit
CTCCTGTGCAGCCTCCGGATTCTCCTTTAGTACTAACTGGATGAACTGGGTCCGCCAAGCTCCGGGGAAGGGG9680.1235157061449064No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9390.11981533891535856No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9330.11904974569545212No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9290.1185393502155145No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8980.11458378524599787No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA8900.1135629942861226No Hit
TCCATGTACCAACCCCCTCTTGTACAGTAATACACAGCCGTGTCCTCGGC8650.11037302253651242No Hit
CTACATAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCA8640.11024542366652801No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8570.10935223157663716No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATC8490.1083314406167619No Hit
GCCTGGGACCCTGGCCAGGTTTCTGCTGGTACCAGGCTATGTAGTTGCTA8460.10794864400680867No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTG8460.10794864400680867No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTCTTC8440.10769344626683987No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8370.10680025417694901No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC8350.10654505643698019No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8260.10539666660712053No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG8180.10437587564724526No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC8150.10399307903729205No Hit
CTGCTACTCTGGCTCCCAGATATTACCGGAGAGATTGTGTTGACGCAGTC8080.10309988694740119No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8010.10220669485751036No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTACCGGAGAGATTGTGTTGACGCAGTCTC7960.1015687005075883No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA7860.10029271180774424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAG200.00789853650.8305828
TCGTATG2750.046.44045640
ATGCCGT2900.045.5582144
CGTATGC3050.045.3503841
TATACAC3750.044.1491283
ATCTCGT2750.043.66264337
CGAGACT3900.043.3478623
ACGAGAC3900.043.3423222
TATCTAT358.70532E-443.0903167
CACGAGA4000.042.24527421
CTTATAC4000.041.40831
CCCACGA4100.041.20701219
CCGTCTT3150.041.09776347
CATCTCG3050.040.4325136
GTATGCC3600.040.41948742
TTATACA4150.039.893792
TCTCCGA4250.039.75264711
ATCTCCG4400.038.3974410
AAAGGGG3800.038.32731668
ATCGCGT451.0349992E-438.1181837
Produced by FastQC (version 0.11.5)