FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783706
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34560.44098169466611203No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24840.316955593041268No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19790.25251816369914226No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA18690.23848228800085747No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16460.2100277399943346No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAATAGCAACTACATAGCCTGGTACC16170.20632737276478677No Hit
TATCAATACTGATGGGGGTAGTGCGACGTACGCGGACTCCGTGGAGGGCCGATTCACCGTCTCCAGAGACAACGC15880.202627005535239No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15870.2024994066652546No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTGATTTGCAGATATA15610.19918183604565998No Hit
GTGTTAATAGCAACTACATAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACAT14750.18820833322700092No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14310.18259398294768703No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13390.17085488690912154No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13150.16779251402949574No Hit
GTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGA12850.16396454792996354No Hit
GTGTATTACTGTACAAGAGGGGGTTGGTACATGGACGTCTGGGGCAAGGG12810.1634541524500259No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCTT12670.1616677682702442No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTGGACTGGAGCCTGAAGATT12480.15924338974054048No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12170.15528782477102382No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGCAGGGCCAGCCAGAGTGTTAATAG11960.15260824850135127No Hit
CTATTATTGTCAATACTATTATAACTCTCAATTCACTTTCGGCCCTGGGA11610.148142288051897No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11570.14763189257195938No Hit
CTCCAGGCACCCTGTCTTTGTCTCCGGGAGAAACAGGCGCCCTCTCCTGC11420.14571790952219327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11330.14456951969233361No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10930.1394655648929573No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10750.13716878523323797No Hit
GTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATCCCA10720.13678598862328475No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10660.13602039540337832No Hit
CCCTATATCTGCAAATCAACAGTCTGAGAGCCGAGGACACGGCTGTGTAT10650.13589279653339392No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10640.1357651976634095No Hit
CTATTATAACTCTCAATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT10580.13499960444350306No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10540.13448920896356542No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10470.13359601687367456No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10440.13321322026372134No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10160.12964045190415793No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10090.12874725981426707No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9790.12491929371473486No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9750.12440889823479723No Hit
CTCCTGTGCAGCCTCCGGATTCTCCTTTAGTACTAACTGGATGAACTGGGTCCGCCAAGCTCCGGGGAAGGGG9660.12326050840493756No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9610.12262251405501554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9450.12058093213526502No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9350.11930494343542093No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9340.11917734456543654No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC9230.11777375699560805No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8820.11254220332624733No Hit
GTATTGATACGTGTGATCCACTCCAGCCCCTTCCCCGGAGCTTGGCGGAC8720.11126621462640328No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA8520.10871423722671512No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC8520.10871423722671512No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8480.1082038417467775No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC8370.10680025417694901No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTCTTC8330.10628985869701138No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8280.10565186434708933No Hit
GCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTACATCCAAGAGGGCCACTGGCATC8180.10437587564724526No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTG8150.10399307903729205No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8130.10373788129732324No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8090.10322748581738561No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8040.10258949146746356No Hit
GCCTGGGACCCTGGCCAGGTTTCTGCTGGTACCAGGCTATGTAGTTGCTA8020.10233429372749475No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG8010.10220669485751036No Hit
GTTATAATAGTATTGACAATAATAGACTGCAAAATCTTCAGGCTCCAGTCCACTGATGGTGAGAGTGAAGT7890.10067550841769744No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT7880.10054790954771305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)