FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835020
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAA50190.6010634475820938No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43200.5173528777753826No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39410.4719647433594405No Hit
CCTATAAATAGTCTGACTGGCCCGGCAAGTGAAGGTGACTCTGTCTCCGACAGATGCAGACAGGGTGGAAGGAGA30980.3710090776268832No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26390.3160403343632488No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24770.29663960144667195No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23870.2858614164930181No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22580.27041268472611435No Hit
GTAGTACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACA22270.26670019879763357No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22190.26574213791286433No Hit
TATTTATTCTCCGTGGGCGTTCGGCCAAGGGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTT21820.2613111063208067No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21780.26083207587842205No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21670.2595147421618644No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGA21670.2595147421618644No Hit
GAATAAATATGATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG21020.2517304974731144No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19860.2378386146439606No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19780.2368805537591914No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19660.23544346243203756No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCTTCACTTGCCG19580.23448540154726835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19140.22921606668103756No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18460.22107254916049918No Hit
GTCGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGACTATTTATAGGTGGTTGGCCTGGTATCAGCAAAAA18210.2180786088955953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17640.21125242509161457No Hit
GTATCATATTTATTCTCCGTGGGCGTTCGGCCAAGGGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCAT17320.20742018155253766No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17290.20706090872074917No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17280.20694115111015307No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15920.19065411606907617No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15790.1890972671313262No Hit
GTGCAGCCACAGTTCGCTTGATGTCCACCCTGGTCCCTTGGCCGAACGCC15510.1857440540346339No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGAGACAGAATTCACTCTTACCATCAGCAGC15100.18083399200019162No Hit
ATATGATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTAAGAGTGAATT14780.17700174846111472No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT14640.17532514191276857No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14600.17484611147038395No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATGTCCACCCTGGTCC14450.17304974731144165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14240.17053483748892242No Hit
GGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14210.17017556465713396No Hit
GAATTCACTCTTACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA14040.16813968527699935No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13990.1675408972240186No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13770.1649062297909032No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13600.16287035041076858No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC13490.16155301669421093No Hit
GGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC13280.1590381068716917No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13140.15736150032334553No Hit
GTGTACTACTGTGCGAGGACTTCTATGAAACCTTCGTCGTGGGCCGCGCG13010.15580465138559557No Hit
CCCTGGTCCCTTGGCCGAACGCCCACGGAGAATAAATATGATACTGTTGG12930.15484659050082633No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12780.15305022634188403No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12730.15245143828890326No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12680.15185265023592248No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12600.15089458935115327No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12530.1500562860769802No Hit
GTCTGAGACAGAATTCACTCTTACCATCAGCAGCCTGCAGCCTGATGATT12420.1487389523604225No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCTTCACTT11960.14323010227299945No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11820.1415534957246533No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11750.14071519245048023No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACC11580.13867931307034562No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCACCTATAA11290.13520634236305717No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT11030.13209264448755717No Hit
CTCCTGATCTATGAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTT10970.13137409882398027No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10380.1243083997988072No Hit
TTCTAAAGTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT10260.12287130847165337No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10210.12227252041867262No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10190.12203300519748031No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10180.12191324758688414No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10160.12167373236569184No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10030.12011688342794184No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG9970.11939833776436493No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9960.11927858015376878No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9920.11879954971138415No Hit
CTCTTACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGC9870.11820076165840339No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9790.11724270077363418No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9770.11700318555244187No Hit
ATGTCCACCCTGGTCCCTTGGCCGAACGCCCACGGAGAATAAATATGATA9640.11544633661469186No Hit
ACTTATTACTGCCAACAGTATCATATTTATTCTCCGTGGGCGTTCGGCCA9540.11424876050873034No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9530.11412900289813417No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC9510.11388948767694186No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9430.11293142679217265No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC9330.11173385068621111No Hit
GCCAAGGGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTC9300.11137457785442265No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9230.11053627458024959No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9220.11041651696965342No Hit
CCCCTAAACTCCTGATCTATGAGGCGTCTACTTTAGAAAGTGGGGTCCCA9090.10885966803190342No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9060.10850039520011498No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC9010.1079016071471342No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8870.10622500059878806No Hit
TCTATGAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGAGACAGAATTCACT8810.10550645493521114No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8780.10514718210342267No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8650.10359033316567268No Hit
CCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCACCTATAAATAGTCTGA8520.10203348422792267No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8460.10131493856434576No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8440.10107542334315345No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8380.10035687767957653No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8370.10023712006898039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)