FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835020
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAA54610.6539963114655936No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41830.5009460851237096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34360.41148715000838304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31280.3746018059447678No Hit
CCTATAAATAGTCTGACTGGCCCGGCAAGTGAAGGTGACTCTGTCTCCGACAGATGCAGACAGGGTGGAAGGAGA28810.3450216761275179No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26440.31663912241622955No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23830.28538238605063354No Hit
TATTTATTCTCCGTGGGCGTTCGGCCAAGGGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTT23260.2785562022466528No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22030.2638260161433259No Hit
GAATAAATATGATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG22020.2637062585327297No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21710.259993772604249No Hit
GTAGTACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGCAGATACA20550.24610188977509523No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20310.2432277071207875No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGA19800.23712006898038368No Hit
GTATCATATTTATTCTCCGTGGGCGTTCGGCCAAGGGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCAT19400.23232976455653756No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19240.2304136427869991No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18480.22131206438169146No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18310.21927618500155685No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18190.21783909367440302No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18130.21712054801082606No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17450.20897703049028768No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16860.2019113314651146No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCTTCACTTGCCG16820.20143230102273002No Hit
GTCGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGACTATTTATAGGTGGTTGGCCTGGTATCAGCAAAAA16720.20023472491676847No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16330.19556417810351845No Hit
GGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG16140.19328878350219156No Hit
GAATTCACTCTTACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA15990.19149241934324926No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15970.19125290412205695No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15750.1886182366889416No Hit
ATATGATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTAAGAGTGAATT15550.18622308447701852No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15480.18538478120284546No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGAGACAGAATTCACTCTTACCATCAGCAGC15420.1846662355392685No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15260.18275011376973008No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15190.181911810495557No Hit
GTGTACTACTGTGCGAGGACTTCTATGAAACCTTCGTCGTGGGCCGCGCG15130.18119326483198006No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14290.17113362554190317No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14160.1695767766041532No Hit
GTCTGAGACAGAATTCACTCTTACCATCAGCAGCCTGCAGCCTGATGATT14140.1693372613829609No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13420.16071471342003785No Hit
GGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC13350.15987641014586476No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13160.15760101554453787No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12760.15281071112069172No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12560.15041555890876865No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12530.1500562860769802No Hit
CTCCTGATCTATGAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTT12520.14993652846638403No Hit
GAGTGAATTCTGTCTCAGACCCACTGCCGCTGAACCTTGATGGGACCCCA12510.1498167708557879No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12410.14861919474982635No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12360.1480204066968456No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11750.14071519245048023No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11490.13760149457498022No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCTTCACTT11360.13604464563723026No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA11340.13580513041603795No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10900.1305357955498072No Hit
CTCTTACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGC10630.12730234006371105No Hit
GTCTCAGACCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGT10610.12706282484251874No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10610.12706282484251874No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10480.12550597590476875No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10380.1243083997988072No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACC10360.12406888457761489No Hit
ACTTATTACTGCCAACAGTATCATATTTATTCTCCGTGGGCGTTCGGCCA10340.1238293693564226No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10200.12215276280807645No Hit
GTGCAGCCACAGTTCGCTTGATGTCCACCCTGGTCCCTTGGCCGAACGCC10140.12143421714449953No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCACCTATAA10090.12083542909151876No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9940.11903906493257647No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATGTCCACCCTGGTCC9750.11676367033124956No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9700.11616488227826878No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9610.1150870637829034No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9460.11329069962396109No Hit
TCTATGAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGAGACAGAATTCACT9440.11305118440276879No Hit
CATCAAGGTTCAGCGGCAGTGGGTCTGAGACAGAATTCACTCTTACCATCAGCAGCCTGCAGCCTGATGATTTTG9410.11269191157098034No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9190.11005724413786495No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9140.10945845608488419No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTAAGAGTGAATTCTGTCTCAGACCCACTGCCG9100.10897942564249959No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9080.10873991042130728No Hit
GTCTTTTACTTTGACTTATGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT9000.10778184953653806No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG8830.10574597015640344No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8820.10562621254580729No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8780.10514718210342267No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8760.10490766688223037No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8710.1043088788292496No Hit
GCCAAGGGACCAGGGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTC8700.10418912121865345No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC8640.10347057555507654No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC8630.10335081794448037No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC8440.10107542334315345No Hit
CCTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCACCTATAAATAGTCTGA8430.1009556657325573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT200.006944262452.51345449
AACGTCG200.007686585751.18184326
AGTCCGA200.007765405351.0499417
TCGACTT200.00776908651.0438216
TAGTCCG200.00776908651.0438216
TCTACTT17500.041.7470
ACAACGA350.001445157938.8718955
TCTGGAG7500.038.1104470
GTGGTAT2800.036.462061
GTATCAA23950.036.09161
TTATGGG6000.034.93456670
GCTATAA400.002761366834.06800522
TATCAAC25050.033.7991182
AGTACAC4750.033.6448023
TCTCGCG2950.032.29697870
AACGCAG26600.031.9689356
ATCAACG26500.031.9497323
CAACGCA26950.031.4256535
TCAACGC27000.031.3580684
GTAGTAC5000.031.3087541
Produced by FastQC (version 0.11.5)