FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318878
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57480.4358249967017419No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48410.3670544204998491No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC46130.34976699891877794No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGATGGAAATACCTACTTGA41800.3169360623196384No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA36220.274627372660701No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36090.27364168634248204No Hit
GTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGAATAAACGA35500.2691681868982575No Hit
ATATATTACTGTGCGAGAGATCCGGCGTTTAGCAGTTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC34320.26022118800980837No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34280.2599178999118948No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31260.2370196485194233No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30900.23429005563820157No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30890.2342142336137232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29460.2233716841133145No Hit
GGTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGAATAAACG28950.219504760864917No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28630.21707845608160875No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC28620.21700263405713036No Hit
GTGATGGAAATACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT28340.21487961737173567No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28130.21328735485768965No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG27690.20995118578064081No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26540.20123165296562687No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG26480.20077672081875655No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25350.19220883205269934No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24630.1867496462902558No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23860.18091135040542036No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23780.1803047742095933No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23300.17666531703463095No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22810.17295003783519022No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA22680.17196435151697126No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22620.17150941937010095No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA21850.1656711234852655No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC21600.16377557287330594No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20780.1575581668660786No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA20400.15467692993590007No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATTACAACATCC20250.15353959956872434No Hit
AGGTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGAATAAACGAACTGTGGCTGCACCATCTGTCTT19750.1497484983448052No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19670.14914192214897815No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTAATGA19410.14717054951254022No Hit
CTCGTACACAGTGATGGAAATACCTACTTGACTTGGTTTCAGCAGAGGCC19030.14428931258236166No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC18650.14140807565218314No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTAATGACTCAGT18200.13799608455065596No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18160.1376927964527424No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTAATGACTCAGTCTCCACTCTCC18010.13655546608556668No Hit
GGACACGACTGGAGAATAAACGAACTGTGGCTGCACCATCTGTCTTCATC17770.13473573749808548No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17640.13375005117986652No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17550.13306765295956108No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16920.128290865417423No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16850.1277601112460743No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA16350.12396901002215518No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16320.12374154394872006No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16190.12275585763050108No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16160.12252839155706594No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15820.11995044272480092No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15800.11979879867584417No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15340.11631098554983857No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA15200.11524947720714122No Hit
CTCCTGTGCAGCCTCTGGATTCAGATTTACTAGCTATTGGATGAGTTGGGTCCGCCAGTCTCCAGGGAAGGGG14970.11350557064413842No Hit
GTTTATTACTGCATGCAAGGTACACACTGGCCCACCTTCGGCCAAGGGAC14950.11335392659518166No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTATTCTCCAGTCGTGTCC14760.1119133081300924No Hit
GTGCAGCCACAGTTCGTTTATTCTCCAGTCGTGTCCCTTGGCCGAAGGTG14300.1084254950040868No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC14000.10615083426973534No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAATACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTC13860.10508932592703799No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13850.10501350390255959No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13840.10493768187808121No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13730.10410363960881902No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTAATGACTCAGTCTCCACT13600.10311795329060004No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13590.10304213126612165No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13580.10296630924164327No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC13580.10296630924164327No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13560.1028146651926865No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13410.10167733482551078No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13190.10000925028698636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG11850.043.36141670
TACGTAT650.001175427940.5390770
GGTATCA11250.039.0282941
GTATCAA27050.035.86081
GTGGTAT4350.032.8626371
TGGTATC4400.032.4707222
ATCAACG30200.032.3261383
TATCAAC30350.031.8313472
ATCAGTG24650.031.80220270
TCAACGC30800.031.696414
CAACGCA31200.031.1833865
AACGCAG31450.031.1541986
GAGTTTA3300.030.9420931
AGAGCAT11600.030.66640970
ACGCAGA32600.030.1595577
CGTTGTC10300.030.00778663
TTGGCGT10450.029.9588859
GCGTTGT10500.029.70234362
CGCAGAG33300.029.4234098
AGAGTAC33000.029.2785211
Produced by FastQC (version 0.11.5)