FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875419
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31480.3595992319106622No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27200.3107083579405976No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCAGAGAGTTCAGCTTCAGGGAGA25820.2949444780156702No Hit
CCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCA22160.253135926910428No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21570.24639629708745184No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20550.2347447336646794No Hit
GTAGTACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAA19500.2227504772000608No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19030.21738161954446955No Hit
CCGTAGTACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCAT17850.20390235989851718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17340.19807657818713098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17200.1964773439918485No Hit
GGGTACTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT16790.19179387241994975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16710.19088002430835976No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16500.18848117301543602No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT16480.18825271098753854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16210.1851684736109223No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT15560.17774345770425362No Hit
GTACTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC15250.17420229627184242No Hit
GGCCAAAGGTGTACCCCAGGGTACTACGGTGCTGACAGTAATAAACTGCA15180.17340267917420116No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14940.17066113483943116No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14260.16289342589091624No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14170.1618653467653775No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT13590.15523994795635004No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13470.15386917578896506No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13110.1497568592868101No Hit
GTATATTACTGTGCGAGGACATATAATTATGATACTAGTGGGTTTTCACCACACGGGTACTGGGGCCAGGGAACC12990.14838608711942508No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12480.1425603054080389No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12200.13936183701747393No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12130.13856221991983267No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11850.1353637515292677No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA11840.13524952051531897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11560.13205105212475396No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11440.130680279957369No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11140.12725334953890652No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGTACCCTGGGGTACACCTTTGGCCAGGGGA11110.12691065649706026No Hit
GTCTCTGGCGGCTCCATCAGCAGTGGTGGGCACTTCTGGAACTGGATCCG11020.12588257737152153No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCCCCTGGCCAAAGGTG10770.12302680202280279No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10700.12222718492516155No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10560.12062795072987906No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10510.1200567956601353No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA10310.11777217538116033No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG10310.11777217538116033No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT10250.11708678929746785No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10190.11640140321377535No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10080.1151448620603391No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10020.11445947597664662No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9860.11263177975346662No Hit
ACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT9830.11228908671162038No Hit
GTTTATTACTGTCAGCACCGTAGTACCCTGGGGTACACCTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAA9660.11034715947449164No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9640.11011869744659414No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9590.10954754237685041No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC9440.10783407716761916No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGGTCC9440.10783407716761916No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9300.10623484297233668No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC9260.10577791891654169No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9050.10337906762361794No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8790.10040906126095048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA252.2481284E-456.7011159
TACGTAT503.70067E-451.25282370
CGTATGC2250.047.61490241
TCGTATG2250.047.56265640
CTTATAC2450.044.6174661
GGTATCA8750.042.553911
GGCAATC2250.041.18293833
CGTTTAC350.001164962740.61396460
ATCTCGT2300.040.4059737
GTATCAA26250.039.951221
ACGAGAC2700.039.22326322
ATGCCGT2750.039.09099644
CACGAGA2750.038.50131621
TCTCCGA2750.038.4859211
CCGTCTT2850.037.8621647
CCCACGA2800.037.80731219
GTATGCC2850.037.63205342
TACACAT2850.037.135545
TTATACA2950.037.0340162
GCCGTCT2950.036.53826546
Produced by FastQC (version 0.11.5)