FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041110
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40090.38506978129112196No Hit
GTATAATAACTGGCCTCGGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT36900.3544294070751409No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34510.33147313924561284No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30660.2944933772608082No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT26260.2522307921353171No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23420.22495221446340924No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22910.2200535966420455No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGGTCAACTCCATGTAGG21510.2066064104657529No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18750.1800962434324903No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17390.16703326257552037No Hit
GTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG17060.16386356869110855No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16980.16309515805246325No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG16550.1589649508697448No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGGTCAGTGGCAGTGGGTCTGGGACAGAGTTCA16550.1589649508697448No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16350.15704392427313155No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16340.1569478729433009No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15910.15281766576058245No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15780.15156899847278385No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15760.15137689581312253No Hit
GTGTATTACTGTGCGAGGGGATATAGCAGTGGCTGGTTCTACTTTGACTC15570.14955192054633995No Hit
AAAGTATACCGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAA15450.14839930458837203No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15380.1477269452795574No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15200.14599802134260548No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15090.1449414567144682No Hit
CTGGTATCCCAGCCAGGGTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14260.1369691963385233No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14010.13456791309275676No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT14000.1344718617629261No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA13510.1297653466012237No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13400.12870878197308644No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13360.1283245766537638No Hit
GTCCCAGACCCACTGCCACTGACCCTGGCTGGGATACCAGTGGCCCTGGT13200.1267877553764732No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCACGGTGAAAGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG13150.1263074987273199No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12900.12390621548155334No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12720.12217729154460144No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12630.12131282957612549No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC12600.12102467558663349No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12590.12092862425680283No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12530.12035231627781887No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12370.11881549500052828No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12100.11622210909510043No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11960.11487739047747116No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11920.1144931851581485No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11870.1140129285089952No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11790.11324451787034992No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCGGTATACTTTTGGCCA11650.11189979925272066No Hit
GTATTACTGTGCGAGGGGATATAGCAGTGGCTGGTTCTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGT11310.10863405403847817No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGGTCAACTCCATGTA10830.1040235902066064No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG10820.10392753887677575No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTATACCGAGGCCAGTTATTATACTG10580.10162230696083988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)