FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507150
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23890.4710637878339741No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17170.3385586118505373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15580.3072069407473134No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCAGAGAGCTCAGCTTCAGGGAAA15460.30484077689046635No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13900.2740806467514542No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13850.2730947451444346No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13050.2573203194321207No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12870.2537710736468501No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11940.23543330375628513No Hit
GTACTACGCTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC11860.23385586118505375No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT11450.22577146800749287No Hit
GCGTAGTACCCTGGGGTACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCAT11390.22458838607906934No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAG11190.22064477965099083No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10260.2023070097604259No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT9780.19284235433303756No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9770.19264517401163364No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9410.18554668244109238No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9360.18456078083407276No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGTAGTACCCTGGGGTACACTTTTGGCCAGGGGA9200.18140589569160998No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9080.17903973183476288No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAGCTACTTAGCCTGGTA8810.17371586315685694No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8670.17095533865720203No Hit
GTATATTACTGTGCGAGGACGTATAACTATGATACTAGTGGTTATTCTCCACAAGGGTACTGGGGCCAGGGAACC8550.16858917480035493No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8540.168391994478951No Hit
CTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAACTGTGGCTGCACCA8480.16720891255052747No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8460.16681455190771963No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8370.1650399290150843No Hit
GTGTTAGGAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC8330.16425120772946858No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8230.16227940451542935No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8120.1601104209799862No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7910.1559696342305038No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTGTT7820.15419501133786848No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7490.14768806073153898No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7260.14315291333924873No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTA7130.14058956916099774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6970.13743468401853495No Hit
GTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCCCCTGGCCAAAAGTG6910.1362516020901114No Hit
GGCCAAAAGTGTACCCCAGGGTACTACGCTGCTGACAGTAATAAACTGCA6840.13487133984028393No Hit
GGGTACTACGCTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT6810.13427979887607217No Hit
GTAGTACCCTGGGGTACACTTTTGGCCAGGGGACCAAACTAGAGATCAAA6690.1319136350192251No Hit
GTCTCTGGCGACTCCATCAGCAGTGATGGGCACTTCTGGAGCTGGATCCG6660.13132209405501333No Hit
TCTATGATGCATCCAACAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT6600.13013901212658976No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6520.12856156955535838No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6480.1277728482697427No Hit
GTTTATTACTGTCAGCAGCGTAGTACCCTGGGGTACACTTTTGGCCAGGGGACCAAACTAGAGATCAAACGAA6310.12442078280587598No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6280.1238292418416642No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC6220.12264615991324065No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6120.12067435669920143No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6030.1188997338065661No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGTTTGGTCC6020.11870255348516218No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT5920.11673075027112294No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5790.11416740609287195No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5760.11357586512866016No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC5760.11357586512866016No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC5660.11160406191462091No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC5630.11101252095040916No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC5620.11081534062900524No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5590.11022379966479345No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5520.10884353741496598No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5520.10884353741496598No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA5510.10864635709356206No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5480.10805481612935028No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA5390.10628019323671498No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5320.1048999309868875No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5280.1041112097012718No Hit
GAGTGTTAGGAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC5260.10371684905846397No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA5250.10351966873706005No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5110.10075914423740509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)