FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720355
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47830.6639781774264079No Hit
GCCTTGGGCTGACCGAGGACGGTCAATGTCGTCCCTCCGCCCAAGAGCCA44380.6160851246954626No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26860.37287170908788025No Hit
CCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATCAGT25220.350105156485344No Hit
CTATTATTGTTGTTCGTCTGTTGGCACCACCTGGCTCTTGGGCGGAGGGACGACATTGACCGTCCTCGGTCAGCC21620.30012979711392296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAATGTCGTCCCTC21350.2963816451610664No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21180.29402169763519376No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20340.28236078044852886No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18740.2601495096167862No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18420.25570725545043765No Hit
CAATAATAGTCAGCCTCATCTTCACCTTGGAGGCCAGAGATGGTCAGGGA17560.24376869737837595No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17400.24154757029520169No Hit
GTGTCCGGGTCTGTTGGACAGTCAGTCACCATCACTTGTACTGGAGCCCG16990.23585593214456763No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16650.23113603709282227No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATCAGTGACTTCTACC15900.22072450389044293No Hit
GGGCACAGGATCCTGGTCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCAC15700.21794809503647505No Hit
GTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTAC15570.216143429281396No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15310.2125340977712378No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15300.2123952773285394No Hit
CACTTGTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTACCAACAACACCCAGGCAGAG15270.21197881600044421No Hit
CAATAAGAGGGCCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCCTCCCTGACCATCT15120.20989650935996834No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14910.2069812800633021No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14770.20503779386552462No Hit
GGCTATGTCTCGTGGTACCAACAACACCCAGGCAGAGCTCCCCAACTCTTCATTTATGATGTCAATAAGAGGGCC14550.20198374412616002No Hit
TCTCTGGCCTCCAAGGTGAAGATGAGGCTGACTATTATTGTTGTTCGTCT14040.19490390154854204No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGT13750.19087810871028868No Hit
ACATTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT13740.1907392882675903No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12970.18005011417981412No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12860.1785230893101318No Hit
CACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12640.17546903957076718No Hit
ACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12410.1722761693887042No Hit
CCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCC12380.17185970806060902No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12350.17144324673251382No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12160.1688056583212444No Hit
GTCAGTCACCATCACTTGTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTACCAACAACA12060.16741745389426047No Hit
GTCAATAAGAGGGCCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCCTCCCTGACCAT12020.1668621721234669No Hit
GGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12010.16672335168076852No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGT11460.15908822733235695No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGCCCTGACTCAGC11430.15867176600426178No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11250.15617299803569074No Hit
GGGTAGAAGTCACTGATGAGACACACCAGTGTGGCCTTGTTGGCTCGAAG11180.155201254936802No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAAT11030.15311894829632614No Hit
GACCGAGGACGGTCAATGTCGTCCCTCCGCCCAAGAGCCAGGTGGTGCCAACAGACGAACAACAATAATAGTCAG11010.15284130741092933No Hit
ATCCTGGTCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTG11000.15270248696823094No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGA10990.15256366652553255No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10970.15228602564013577No Hit
CCCCTGGGGCCCTCTTATTGACATCATAAATGAAGAGTTGGGGAGCTCTGCCTGGGTGTTGTTGGTACCACGAGA10830.1503425394423583No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAATGTCGTCCCTCCGCCCAAGAGCCAGGTGGTGC10260.14242977420854996No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10230.1420133128804548No Hit
CTCTTATTGACATCATAAATGAAGAGTTGGGGAGCTCTGCCTGGGTGTTGTTGGTACCACGAGACATAGCCAGAA10180.14131921066696282No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10050.13951454491188373No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10010.13895926314109014No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9970.1384039813702966No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACACCAGTGTGGC9900.13743223827140785No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9500.13187942056347218No Hit
GTACCAACAACACCCAGGCAGAGCTCCCCAACTCTTCATTTATGATGTCA9410.13063003657918665No Hit
GACCATCTCTGGCCTCCAAGGTGAAGATGAGGCTGACTATTATTGTTGTT9380.13021357525109148No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCC9370.1300747548083931No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG8920.12382783488696546No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT8760.12160670780379118No Hit
GAGATGGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAGAGAAGCGATC8690.12063496470490244No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAGAGAAG8530.11841383762172816No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGG8520.11827501717902979No Hit
CCCCAACTCTTCATTTATGATGTCAATAAGAGGGCCCCAGGGGTCCCTGA8480.11771973540823623No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8480.11771973540823623No Hit
TCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGCCCTGACTCAGCCT8450.11730327408014105No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8430.11702563319474425No Hit
AGACACACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8350.11591506965315712No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCACCATCA8210.11397158345537964No Hit
GAGCCAGGTGGTGCCAACAGACGAACAACAATAATAGTCAGCCTCATCTT8090.11230573814299893No Hit
GTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8000.1110563541587134No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7980.11077871327331663No Hit
GCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCACCATCACTTGTA7980.11077871327331663No Hit
AGGCCACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7960.11050107238791984No Hit
CTTATTGACATCATAAATGAAGAGTTGGGGAGCTCTGCCTGGGTGTTGTT7870.10925168840363432No Hit
CTCCAGTACAAGTGATGGTGACTGACTGTCCAACAGACCCGGACACTGAG7810.10841876574744395No Hit
ACCTGGCTCTTGGGCGGAGGGACGACATTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC7780.10800230441934879No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7770.1078634839766504No Hit
ATGTCGTCCCTCCGCCCAAGAGCCAGGTGGTGCCAACAGACGAACAACAA7730.10730820220585682No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCAC7730.10730820220585682No Hit
GTACAAGTGATGGTGACTGACTGTCCAACAGACCCGGACACTGAGCGAGG7690.10675292043506328No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7670.10647527954966649No Hit
CATCTTCACCTTGGAGGCCAGAGATGGTCAGGGAGGCCGTGTTGTCAGACTTGGAGCCAGAGAAGCGATCAGGGA7650.1061976386642697No Hit
GTGAAGATGAGGCTGACTATTATTGTTGTTCGTCTGTTGGCACCACCTGGCTCTTGGGCGGAGGGACGACATTGA7610.10564235689347615No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACACCAGTGTGGCCTTGTTGGCTCGAAG7450.10342122981030187No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGCCCTGACT7440.10328240936760347No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7420.1030047684822067No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7410.1028659480395083No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC7310.10147774361252439No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACACCAGTGTGGCC7310.10147774361252439No Hit
GAGTTGGGGAGCTCTGCCTGGGTGTTGTTGGTACCACGAGACATAGCCAG7260.10078364139903243No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC7250.10064482095633402No Hit
GTTGGACAGTCAGTCACCATCACTTGTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTAC7240.10050600051363563No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG7230.10036718007093726No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGATGGTTCATGCCTAAGAGTGGTGGCACAAACTATGCCCGGCAGTTC7210.10008953918554046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1700.073.4603470
GGTATCA4800.053.364651
TCGTATG1250.049.6602640
ATCTCGT1250.046.77670337
GCTAATC1250.046.63319833
CGTATGC1350.046.02044741
AATCTCG1350.043.293636
CCGTCTT1450.043.18915647
TCTCGTA1400.041.79707338
TAATCTC1400.041.7182335
CCCACGA1500.040.96413819
TCTCCGA1600.040.54024511
AATGGTA1805.456968E-1240.16691270
ATCTCCG1550.039.6454710
CGTCTTC1600.039.19840648
GTATCAA13600.039.1759341
ATGCCGT1600.039.0104644
CTAATCT1500.038.9098334
TGACCTA451.0651339E-437.9323929
ACACATC1900.037.737996
Produced by FastQC (version 0.11.5)