FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720355
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44540.6183062517786369No Hit
GCCTTGGGCTGACCGAGGACGGTCAATGTCGTCCCTCCGCCCAAGAGCCA35960.49919831194341674No Hit
CCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATCAGT25310.35135454046962955No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25240.35038279737074085No Hit
CTATTATTGTTGTTCGTCTGTTGGCACCACCTGGCTCTTGGGCGGAGGGACGACATTGACCGTCCTCGGTCAGCC22890.31775999333661875No Hit
GTGTCCGGGTCTGTTGGACAGTCAGTCACCATCACTTGTACTGGAGCCCG21240.2948546202913841No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20970.2911064683385275No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19990.27750206495408514No Hit
GGGCACAGGATCCTGGTCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCAC19230.26695171130900736No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19170.266118788652817No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAATGTCGTCCCTC18620.25848366430440545No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18300.2540414101380569No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18010.25001561729980354No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGT17300.24015936586821773No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16600.23044193487933032No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATCAGTGACTTCTACC15380.21350584087012656No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15380.21350584087012656No Hit
TCTCTGGCCTCCAAGGTGAAGATGAGGCTGACTATTATTGTTGTTCGTCT15240.21156235467234905No Hit
CAATAAGAGGGCCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCCTCCCTGACCATCT15120.20989650935996834No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14790.20531543475092143No Hit
CACTTGTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTACCAACAACACCCAGGCAGAG14730.2044825120947311No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14710.20420487120933428No Hit
CAATAATAGTCAGCCTCATCTTCACCTTGGAGGCCAGAGATGGTCAGGGA14530.2017061032407632No Hit
GGCTATGTCTCGTGGTACCAACAACACCCAGGCAGAGCTCCCCAACTCTTCATTTATGATGTCAATAAGAGGGCC14430.20031789881377934No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGCCCTGACTCAGC14300.19851323305870022No Hit
GTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTAC13970.19393215844965328No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13730.1906004678248919No Hit
ATCCTGGTCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTG13730.1906004678248919No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13360.1854641114450514No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13250.18393708657536909No Hit
ACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12910.17921719152362375No Hit
GTCAGTCACCATCACTTGTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTACCAACAACA12560.17435847602918006No Hit
GGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12300.17074914451902187No Hit
ACATTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT12300.17074914451902187No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11810.16394694282680067No Hit
CCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCC11580.16075407264473765No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11560.1604764317593409No Hit
CACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11510.15978232954584892No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11500.15964350910315053No Hit
GTCAATAAGAGGGCCCCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGACAACACGGCCTCCCTGACCAT11220.15575653670759557No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGT10930.15173074386934218No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGA10910.15145310298394543No Hit
TCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGCCCTGACTCAGCCT10580.1468720283748985No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10510.14590028527600973No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10320.1432626968647403No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10150.14090274933886765No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCAC10070.1397921857972805No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAATGTCGTCCCTCCGCCCAAGAGCCAGGTGGTGC9940.1379875200422014No Hit
GACCATCTCTGGCCTCCAAGGTGAAGATGAGGCTGACTATTATTGTTGTT9860.13687695650061427No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCACCATCA9850.1367381360579159No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9800.13604403384442393No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGCCCTGACT9720.13493347030283678No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAAT9640.13382290676124967No Hit
GGGTAGAAGTCACTGATGAGACACACCAGTGTGGCCTTGTTGGCTCGAAG9630.13368408631855125No Hit
GCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCACCATCACTTGTA9590.13312880454775772No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACACCAGTGTGGC9510.13201824100617057No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT9220.1279924481679172No Hit
GACCGAGGACGGTCAATGTCGTCCCTCCGCCCAAGAGCCAGGTGGTGCCAACAGACGAACAACAATAATAGTCAG9130.12674306418363168No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9010.12507721887125098No Hit
CCCCAACTCTTCATTTATGATGTCAATAAGAGGGCCCCAGGGGTCCCTGA8980.12466075754315581No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC8780.12188434868918797No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8760.12160670780379118No Hit
GGATCCTGGTCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTC8720.12105142603299761No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGTCTCAGTC8680.12049614426220404No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC8470.11758091496553782No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8390.1164703514239507No Hit
AGACACACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8340.11577624921045872No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGG8250.11452686522617321No Hit
GTACCAACAACACCCAGGCAGAGCTCCCCAACTCTTCATTTATGATGTCA8180.11355512212728447No Hit
GTTGGACAGTCAGTCACCATCACTTGTACTGGAGCCCGCGAGAATATTGGTCGTTCTGGCTATGTCTCGTGGTAC8110.11258337902839571No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTGTTGGACAGTCAGTCACCATCACTTGTACTGGAGCC8050.11175045637220538No Hit
CCCCTGGGGCCCTCTTATTGACATCATAAATGAAGAGTTGGGGAGCTCTGCCTGGGTGTTGTTGGTACCACGAGA7950.11036225194522147No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7860.10911286796093593No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7850.10897404751823753No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGTCTCAGTCTGC7830.10869640663284076No Hit
ACCTGGCTCTTGGGCGGAGGGACGACATTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC7760.10772466353395202No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7670.10647527954966649No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG7580.10522589556538095No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7580.10522589556538095No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7570.10508707512268257No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAATGTCGTCCCTCCGCCCAAGAG7570.10508707512268257No Hit
GTGAAGATGAGGCTGACTATTATTGTTGTTCGTCTGTTGGCACCACCTGGCTCTTGGGCGGAGGGACGACATTGA7510.10425415246649221No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCC7470.10369887069569864No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC7390.1025883071541115No Hit
GAGCCAGGTGGTGCCAACAGACGAACAACAATAATAGTCAGCCTCATCTT7330.10175538449792117No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7330.10175538449792117No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG7300.101338923169826No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACCATGGCCTGGGCT7270.10092246184173082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1600.044.73541
GGTATCA6250.041.991631
TGGTATC1850.038.657842
GTATCAA14000.037.249071
GACAGCC1850.036.8476941
GAGTTTA1450.035.259431
CAACAAT14650.032.67164670
TATCAAC16400.032.1868062
TATTGGG450.004924020730.2717482
AGTTTAT1600.029.7987542
ACTGGTG25500.029.3449370
ATCAACG17850.028.9917743
TTATTCA1650.028.8897425
AACGCAG18300.028.654936
CAACGCA18150.028.5145515
AGTTCGG950.006739506528.38485770
TCAACGC18400.028.3102074
TGCCCGT500.00802518227.3910131
CGAACTA500.008179408527.28435524
GACAGGT1254.7184585E-927.2672921
Produced by FastQC (version 0.11.5)