FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075313
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25510.2372332520856718No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19930.18534138432251818No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19860.1846904110710091No Hit
GACTTAGGACGGTCAGCGTGGTCCCTGCGCCGAAGAGGGGATGATTACCA19860.1846904110710091No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18620.1731588849014194No Hit
CCTATGTTCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCCGGAGAGACG17610.16376627084393103No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCTG17350.16134837019546866No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCAGTGCACAGGCTCTG16510.15353669117735952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15640.14544602362288933No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15260.1419121688289828No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCTGCGCCGAAGAGGGGATGATTACCACGGACATCCCACAC14610.1358674172078269No Hit
GCCCTTAGGGATCCCTGAGCGCTTCTCTGGCTCCAACTCTGGGAACACGG14480.13465846688359576No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14180.13186858151998535No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCAGTGCACAGGCTCTGTGAACTCCTATGTTCTGACT13540.12591682607761648No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13220.12294094835643204No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13160.12238297128370995No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12830.1193140973837385No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12800.11903510884737746No Hit
GTGCACAGGCTCTGTGAACTCCTATGTTCTGACTCAGCCACCGTCGGTGT12710.11819814323829433No Hit
CCTGTGGGGGAGACAAGATTGGAAGTAAAAGTGTCCAGTGGTACCAGCAG12510.11633821966255406No Hit
GGCTCTGTGAACTCCTATGTTCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCCGGAGAGACGGCCACGATCAC12390.1152222655171099No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12030.11187440308077741No Hit
GTCTGGGCCTGAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCT11820.10992148332625011No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11650.1083405482868709No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11490.10685260942627868No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11360.1056436591020475No Hit
GGGTTTATTGAGTGTAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11310.10517867820811243No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC11080.10303976609601112No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11060.1028537737384371No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAAGCGCTCAGGGATCCCTAAGGGCCGCTCGGAGTCATCATGGA10840.10080785780512279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.041.8060151
CGTATGC1650.039.88391541
GTATCAA29100.037.4494821
TCGTATG1700.034.6017640
ACTCACG501.9484619E-434.2324429
AGGGGGG3950.033.04873770
GGCGAAT2100.032.6708532
TAAGGCG2250.031.95027529
GCGAATC1950.031.68186833
GTGGTAT3350.031.612951
ATCAACG34500.031.4785563
GTATGCC2100.031.36990442
ATCTCCG2300.031.18738710
TATCAAC34800.031.0109162
TCAACGC34950.030.9755344
ATCTCGT1900.030.82875337
ACTAAGG2450.030.71928827
TCTCGTA1800.030.66034538
AACGCAG35550.030.266246
GGTACTA1102.7039277E-829.12277660
Produced by FastQC (version 0.11.5)