FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075313
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25010.23258344314632112No Hit
GTGTAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24220.22523674502214702No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19300.17948262505893633No Hit
GACTTAGGACGGTCAGCGTGGTCCCTGCGCCGAAGAGGGGATGATTACCA18840.1752048008347337No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18430.17139195750446615No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17460.16237132816212582No Hit
CCTATGTTCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCCGGAGAGACG16330.15186275995919327No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCTG15190.1412611955774737No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCTGCGCCGAAGAGGGGATGATTACCACGGACATCCCACAC14580.1355884286714659No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCAGTGCACAGGCTCTGTGAACTCCTATGTTCTGACT14470.13456547070480873No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14340.13335652038057755No Hit
GCCCTTAGGGATCCCTGAGCGCTTCTCTGGCTCCAACTCTGGGAACACGG14020.13038064265939311No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13970.12991566176545805No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCAGTGCACAGGCTCTG13920.129450680871523No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13920.129450680871523No Hit
CCTGTGGGGGAGACAAGATTGGAAGTAAAAGTGTCCAGTGGTACCAGCAG12820.1192211012049515No Hit
GGCTCTGTGAACTCCTATGTTCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCCGGAGAGACGGCCACGATCAC12610.11726818145042421No Hit
GGGTTTATTGAGTGTAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12450.11578024258983198No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12370.11503627315953588No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAAGCGCTCAGGGATCCCTAAGGGCCGCTCGGAGTCATCATGGA12310.11447829608681379No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12210.11354833429894366No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12140.11289736104743457No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11880.1104794603989722No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11790.10964249478988908No Hit
GTGCACAGGCTCTGTGAACTCCTATGTTCTGACTCAGCCACCGTCGGTGT11470.10666661706870464No Hit
GTCTGGGCCTGAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCT11300.1050856820293254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG703.3450175E-546.16140770
GTGGTAT5050.037.1594121
GTATCAA30750.035.2841381
GGTATCA13050.035.034171
TTATCGT400.002752156534.092124
CTAGTAC400.002752156534.092123
TGGTATC5950.031.5137252
TATCAAC34350.031.2635172
AACGCAG34250.031.061966
TCAACGC34700.030.8499324
ATCAACG34900.030.7708263
ACGCAGA36300.029.4003377
CGCAGAG37300.028.5207148
CAGAGTA38450.027.75636710
AGAGTAC38550.027.68436611
CAACGCA38600.027.6485045
GCAGAGT38950.027.3987859
ACGAACC2001.8189894E-1224.5918152
TATTGCG750.001818107123.36590650
GACAGGT2700.022.7460631
Produced by FastQC (version 0.11.5)