FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171795
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGAACCACTCAGGCTGCTGTCATAAGACTGGCAGTAATAATCACCC67900.5794528906506684No Hit
GTTCAGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC59920.5113522416463631No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42160.35978989499016467No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCC40420.34494088129749656No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTTCACTGGTACCACCAACTTCCGGGAACAGC37980.32411812646409993No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36950.31532819307131366No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33710.2876783055056559No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31210.2663435157173396No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCCTGAACCACTC30610.26122316616814373No Hit
GTCATAAGACTGGCAGTAATAATCACCCTCATCCTCAGCCTGGAGCCCAG30500.2602844354174578No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29850.25473739007249563No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28830.2460327958388626No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28250.24108312460797326No Hit
GGTTATGATGTTCACTGGTACCACCAACTTCCGGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGG28050.2393763414249079No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27150.23169581710111412No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26430.22555139764207904No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25520.21778553415913193No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25160.21471332442961438No Hit
GTCTTATGACAGCAGCCTGAGTGGTTCAGGAGTGTTCGGCGGAGGGACCA25130.2144573069521546No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT23860.20361923373968996No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22740.1940612479145243No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22570.1926104822089188No Hit
GGACAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTTCACTGGTACCAC22560.19252514304976553No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22430.19141573398077308No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22170.1891969158427882No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22050.18817284593294903No Hit
GTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCCGGAAGTTGGTGGTA21460.1831378355429064No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21340.18211376563306722No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21160.18057766076830845No Hit
ATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG20820.17767612935709742No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20580.1756279895374191No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC19980.1705076399882232No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19970.17042230082906992No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGT19950.1702516225107634No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19110.16308313314188916No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC18000.15361048647587675No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17820.152074381611118No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17670.15079429422381901No Hit
GTAATACAAGGCGGTGTCCTCAATTCTCAGACTGTTCATTTGCAAATACA17520.14951420683652003No Hit
CCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATC17370.14823411944922107No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCCTGAACCACTCA17280.1474660670168417No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17230.14703937122107535No Hit
CCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC16930.14447919644647741No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16380.13978554269304785No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16110.1374813853959097No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15840.13517722809877156No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15580.13295840996078667No Hit
GTGCAGGAGATGGTGACCCTCTGTCCTGGGGCCCCAGACACTGAGGGCGG15550.13270239248332685No Hit
GTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCAG15470.13201967921010074No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15420.13159298341433442No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCACCATCTCCTG15340.1309102701411083No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15210.12980086107211586No Hit
GGATGAGGGTGATTATTACTGCCAGTCTTATGACAGCAGCCTGAGTGGTT15200.1297155219129626No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15090.1287767911622767No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15010.12809407788905056No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15000.1280087387298973No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC14970.1277527212524375No Hit
GCTCCAACATCGGGGCAGGTTATGATGTTCACTGGTACCACCAACTTCCG14770.1260459380693722No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14740.1257899205919124No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC14510.12382711993138731No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT14500.12374178077223406No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT14430.12314440665816119No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGTCTTAT14270.12177898011170894No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14150.12075491020186978No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT14140.12066957104271653No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13870.11836541374557837No Hit
GTTTGGGGGCTGTTCCCGGAAGTTGGTGGTACCAGTGAACATCATAACCT13760.11742668299489245No Hit
GCCCCCAAACTCCTCATCTATGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13760.11742668299489245No Hit
GTTATGATGTTCACTGGTACCACCAACTTCCGGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGGC13690.1168293088808196No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13680.11674396972166633No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC13640.11640261308505327No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG13440.11469582990198798No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13320.11367175999214879No Hit
GTATTACTGTGCAAAAGGATCGGGAGATAGTGTGATTCGGGGAGCCACCGATTACTGGGGCCAGGGAACCCTGGT13080.11162362017247045No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12940.11042887194432473No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12720.10855141044295291No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12630.10778335801057352No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12610.107612679692267No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12540.10701530557819415No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12490.10658860978242782No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCCTGAACCA12430.10607657482750822No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGTCCTGGGGCCCCAGACA12400.10582055735004843No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12320.1051378440768223No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG12260.10462580912190271No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12110.10334572173460374No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11930.10180961686984498No Hit
GATTATTACTGCCAGTCTTATGACAGCAGCCTGAGTGGTTCAGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTC11920.10172427771069173No Hit
TTGTATTACTGTGCAAAAGGATCGGGAGATAGTGTGATTCGGGGAGCCAC11900.10155359939238517No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT11890.10146826023323192No Hit
GTAATAATCACCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG11770.10044419032339273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.052.8416251
AGGGGGG4300.048.1492570
TCGTATG2850.042.36236640
ACGCGTA2850.041.99331727
TATACAC3100.041.8837173
CGCGTAG2900.041.283428
GACGCGT2900.041.25872426
TCTCGTA2700.040.80043438
CTTATAC2950.040.5663531
AATCTCG2750.039.9915536
GCGTAGT3000.039.9260529
AGACGCG3000.039.8766225
CGTATGC3050.039.6391541
CGAGACG3050.039.21286423
TTATACA3250.038.899292
ATCTCGT2850.038.61983537
GTATCAA25950.038.2103081
GCCGTCT3200.038.02052346
ACGAGAC3150.037.96477522
ATGCCGT3200.037.9151844
Produced by FastQC (version 0.11.5)