FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436625
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATGTTAAGAAACA21350.48897795591182364No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTAATGGCCAGGGAGG18260.41820784425994845No Hit
GATTATTACTGTGCAGCATGGGATGACAGCTTGACTGGTCCGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTA17420.39896936730604066No Hit
GTGTATTACTGTGCGAGAGGTTGGATGACTACAACCTTTGACTACTGGGG13250.30346407099914113No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCACCGTCTGGGACCC12230.28010306326939594No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11650.2668193529916977No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11410.26132264529058113No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCACCGTCT10170.2329229888348125No Hit
ACCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTC9580.21941024906956771No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9540.2184941311193816No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9200.21070712854279988No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT8490.19444603492699686No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8320.19055253363870597No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGC8110.18574291440022903No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7820.1791010592613799No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7770.17795591182364728No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT7650.17520755797308904No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7620.17452046951044947No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG7470.17108502719725163No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7460.1708559977097051No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7260.1662754079587747No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7260.1662754079587747No Hit
CAGTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATGTTAAGAAA7220.1653592900085886No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7200.16490123103349558No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7110.16283996564557687No Hit
GGTCTGGAGTGGGTCTCAGTTATTTACAGCGGCGATACCACATATTATGC7080.1621528771829373No Hit
GTGCTGACTCAGCCACCCTCACCGTCTGGGACCCCCGGGCAGAGGGTCAC7040.16123675923275121No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCT6960.15940452333237906No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC6930.15871743486973947No Hit
GGCCATTAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG6920.15848840538219294No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6900.15803034640709993No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACTACATGAGCT6850.1568851989693673No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6690.15322072716862295No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6680.15299169768107643No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6590.15093043229315775No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6560.15024334383051818No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6560.15024334383051818No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG6490.14864013741769252No Hit
GTATTACTGTGCGAGAGGTTGGATGACTACAACCTTTGACTACTGGGGCC6270.14360148869166905No Hit
GGTCTACCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACTACATGAG6260.14337245920412253No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6100.1397079874033782No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC5920.1355854566275408No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA5850.13398225021471513No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCTTGACTGGTCCGGTGTTCGGCGGCGGGAC5840.1337532207271686No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5800.13283710277698255No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5790.13260807328943602No Hit
CTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTC5780.13237904380188947No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATTTA5780.13237904380188947No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5730.1312338963641569No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAG5650.12940166046378473No Hit
TCCTCAGACTGGAGCCCACTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGACC5560.127340395075866No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5520.12642427712567994No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG5390.12344689378757515No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5370.1229888348124821No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5290.12115659891210995No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5280.12092756942456342No Hit
CCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCAGT5200.11909533352419124No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG5120.11726309762381906No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT5110.11703406813627255No Hit
GTCAAGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGA5090.11657600916117951No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT5050.11565989121099342No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCAGGGTTCCCCGG5030.11520183223590037No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5030.11520183223590037No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC5000.11451474377326079No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGCAGTTTGGGCTGAGCTGGG4990.1142857142857143No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4960.11359862582307471No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC4920.11268250787288864No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA4920.11268250787288864No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4920.11268250787288864No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAATTGGTACCAGCAGCTCCCAGGAACGGCCCC4910.11245347838534211No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC4880.11176638992270253No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT4860.11130833094760952No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC4820.11039221299742341No Hit
GAAGTAATACTGTAAATTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA4820.11039221299742341No Hit
GAACACAGAGGACTCGCCATGCAGTTTGGGCTGAGCTGGGTTTTCCTTGT4790.10970512453478386No Hit
GCACTGAACACAGAGGACTCGCCATGCAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCAATTTTAAAAGGTGTC4790.10970512453478386No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4780.10947609504723733No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4720.1081019181219582No Hit
CTCCAGACCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGTAGTAGTTGC4690.10741482965931864No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC4650.10649871170913254No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4650.10649871170913254No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4640.10626968222158602No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4630.10604065273403951No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4620.10581162324649299No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4610.10558259375894646No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4530.10375035785857428No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT4490.1028342399083882No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT4450.10191812195820213No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG4380.10031491554537647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA100.00995387129.4249970
AGGGGGG1750.073.9571470
GATCCAC200.00658618353.21664454
ACGATTA200.00764382451.2499733
TTTAGTC200.007787478651.00919725
GGGTAGT200.007801600350.9858318
CGTATGC1450.050.07654641
ACGTAGA1500.049.9099727
ATGCCGT1500.048.7023944
TCGTATG1500.048.31725740
CGTAGAG1550.048.3055128
AGACGTA1600.046.7584325
CGAGCCC1600.046.73700715
TCTCCGA1600.046.73700711
GGTATCA3750.046.2695471
CTTGTAA306.782984E-445.3259282
TATACAC1650.045.3259243
GAATCTC1450.044.8837735
CTTATAC1750.044.7142641
ATCTCCG1700.043.9877710
Produced by FastQC (version 0.11.5)