FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436625
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATGTTAAGAAACA18380.4209561981105068No Hit
GATTATTACTGTGCAGCATGGGATGACAGCTTGACTGGTCCGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTA17870.40927569424563415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15710.3598053249355854No Hit
GTGTATTACTGTGCGAGAGGTTGGATGACTACAACCTTTGACTACTGGGG13400.30689951331233895No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCACCGTCTGGGACCC12420.28445462353277984No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11700.26796450042943026No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11190.2562839965645577No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCACCGTCT9790.22421986830804466No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTAATGGCCAGGGAGG9540.2184941311193816No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8870.20314915545376466No Hit
ACCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTC8800.201545949040939No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8320.19055253363870597No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8230.18849126825078727No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8070.18482679645004294No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT7950.1820784425994847No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGC7910.1811623246492986No Hit
GTGCTGACTCAGCCACCCTCACCGTCTGGGACCCCCGGGCAGAGGGTCAC7870.1802462066991125No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG7870.1802462066991125No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC7500.17177211565989123No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7440.1703979387346121No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7310.1674205553965073No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCGGACCAGTCAAGCTGTCATCCCATG6860.1571142284569138No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACTACATGAGCT6840.1566561694818208No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6840.1566561694818208No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6810.15596908101918122No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG6790.15551102204408818No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6660.1525336387059834No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6560.15024334383051818No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6550.15001431434297166No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCT6540.14978528485542514No Hit
GTATTACTGTGCGAGAGGTTGGATGACTACAACCTTTGACTACTGGGGCC6510.14909819639278557No Hit
GGTCTGGAGTGGGTCTCAGTTATTTACAGCGGCGATACCACATATTATGC6510.14909819639278557No Hit
GACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCGGACCAGTCAAG6480.148411107930146No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6170.14131119381620383No Hit
CAGTAATACACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATGTTAAGAAA6170.14131119381620383No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6080.13924992842828512No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA6060.1387918694531921No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6050.13856283996564558No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6000.13741769252791297No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5940.13604351560263384No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATTTA5850.13398225021471513No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5820.1332951617520756No Hit
GGCCATTAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG5800.13283710277698255No Hit
CTCCAGACCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGTAGTAGTTGC5790.13260807328943602No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5660.12963068995133123No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT5620.12871457200114514No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5600.1282565130260521No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5570.12756942456341255No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC5540.12688233610077296No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5480.12550815917549385No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAG5470.12527912968794733No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5430.12436301173776124No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5350.12253077583738907No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5280.12092756942456342No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG5180.11863727454909818No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5160.11817921557400515No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT5150.11795018608645863No Hit
CTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTC5130.11749212711136559No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCTTGACTGGTCCGGTGTTCGGCGGCGGGAC5070.11611795018608645No Hit
GGTCTACCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCGTCAGTAGCAACTACTACATGAG5020.11497280274835385No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC4940.11314056684798167No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4910.11245347838534211No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC4860.11130833094760952No Hit
GAACACAGAGGACTCGCCATGCAGTTTGGGCTGAGCTGGGTTTTCCTTGT4860.11130833094760952No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT4840.11085027197251646No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4760.10901803607214428No Hit
CCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCAGT4760.10901803607214428No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC4710.10787288863441168No Hit
GAAGTAATACTGTAAATTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA4620.10581162324649299No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4610.10558259375894646No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAATTGGTACCAGCAGCTCCCAGGAACGGCCCC4550.10420841683366734No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4540.1039793873461208No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGCAGTTTGGGCTGAGCTGGG4530.10375035785857428No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT4470.10237618093329516No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4450.10191812195820213No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC4440.1016890924706556No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT4430.10146006298310907No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACCAGGGTTCCCCGG4430.10146006298310907No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT4420.10123103349556255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)