FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865936
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31930.36873394800539533No Hit
ATATTATAGTACTCCGTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT31700.36607786256721053No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30240.34921749413351566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21390.24701594575118718No Hit
GACCAGGACAGCCTCCTAAGTTGCTCATTAGCTGGGCATCTACCCGGGAA20500.2367380499251677No Hit
CTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGTAGGCTACTGATGGTGAGAGTGAAATTTGTC19490.22507437039226919No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19400.2240350326121099No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17690.2042876147890837No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16950.19574194859666305No Hit
ACTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTTGTGGTTGAAGAAAACGCT16600.1917000794515992No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16580.19146911550045267No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16360.18892851203784114No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16080.18569501672179006No Hit
CTCCTAAGTTGCTCATTAGCTGGGCATCTACCCGGGAATCAGGGGTCCCT15630.1804983278209937No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15250.17611001274921012No Hit
TTATAGTACTCCGTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT14530.16779531050793592No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14510.16756434655678942No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14040.16213669370484654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13780.159134162339942No Hit
GTAGTACACGGCCGTGTCTGCGGCGGTCACAGAGGCCAGCTTCAGGGAGA13330.1539374734391456No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTTGTGGTTG13210.15255168973226657No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGCGTTTTCTT13100.1512813880009608No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13100.1512813880009608No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13010.15024205022080153No Hit
GTCTCTGATGGGTCCATCAGTGGTTTCTACTGGACCTGGATCCGGCAGTC12980.14989560429408177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12790.14770144675819No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12550.14492987934443194No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGTTGCTCATTAGCTGGGCATCTACCCGGGAATCAGGGGTCCCTGA11920.137654514883317No Hit
ATAGTACTCCGTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGA11670.1347674654939857No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11480.1325733079580939No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11380.13141848820236138No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGCGTTTTCTTCAACCACAA10970.12668372720385804No Hit
GTACTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGTAG10910.1259908353504185No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10490.12114059237634191No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGACCCCTGGCCAAAAGTA10480.12102511040076865No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10450.12067866447404889No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10130.11698324125570482No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9960.11502004767095951No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9610.11097817852589568No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9600.11086269655032242No Hit
ATTCAGTGGCAGCGGGTCTGGGACAAATTTCACTCTCACCATCAGTAGCC9600.11086269655032242No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC9570.11051625062360267No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGCGTTTTCTTCAACCACAACAGTAAGAACTA9450.10913046691672364No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9340.10786016518541787No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9210.10635889950296558No Hit
GTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGAACTGTGGCTG9160.10578148962509931No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8880.10254799430904823No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAAATTTCACTCTCAC8790.10150865652888898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)