FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865936
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31760.36677075442065005No Hit
ATATTATAGTACTCCGTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT31510.3638837050313187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24410.28189150237430943No Hit
CTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGTAGGCTACTGATGGTGAGAGTGAAATTTGTC23410.27034330481698415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20700.23904768943663274No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19570.22599822619685522No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19130.22091701927163207No Hit
GACCAGGACAGCCTCCTAAGTTGCTCATTAGCTGGGCATCTACCCGGGAA17230.19897544391271413No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17120.19770514218140833No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16920.1953955026699433No Hit
ACTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTTGTGGTTGAAGAAAACGCT15880.18338537721032502No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15830.18280796733245874No Hit
CTCCTAAGTTGCTCATTAGCTGGGCATCTACCCGGGAATCAGGGGTCCCT14630.16895013026366845No Hit
TTATAGTACTCCGTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT14050.1622521756804198No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13800.15936512629108848No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13630.1574019327063432No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13100.1512813880009608No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13100.1512813880009608No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12960.14966464034293528No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12890.1488562665139225No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12790.14770144675819No Hit
GTAGTACACGGCCGTGTCTGCGGCGGTCACAGAGGCCAGCTTCAGGGAGA12680.1464311450268842No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12240.1413499381016611No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12200.14088801019936809No Hit
GTACTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGTAG12130.1400796363703553No Hit
GTCTCTGATGGGTCCATCAGTGGTTTCTACTGGACCTGGATCCGGCAGTC12010.13869385266347628No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTTGTGGTTG11670.1347674654939857No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11520.13303523586038693No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGCGTTTTCTT11350.13107204227564162No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11340.13095656030006836No Hit
ATAGTACTCCGTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGA11290.1303791504222021No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10920.12610631732599176No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGACCCCTGGCCAAAAGTA10670.12321926793666044No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAGTTGCTCATTAGCTGGGCATCTACCCGGGAATCAGGGGTCCCTGA10630.12275734003436743No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10180.11756065113357109No Hit
TCTTCAGCCTGTAGGCTACTGATGGTGAGAGTGAAATTTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10170.11744516915799781No Hit
GAGTACTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGT10070.11629034940226529No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9970.11513552964653277No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGCGTTTTCTTCAACCACAA9910.11444263779309324No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9830.11351878198850723No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9790.11305685408621421No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9210.10635889950296558No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9080.1048576338205133No Hit
ATTCAGTGGCAGCGGGTCTGGGACAAATTTCACTCTCACCATCAGTAGCC9000.10393377801592726No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8990.10381829604035403No Hit
AAAGTATACGGAGTACTATAATATTGCTGACAGTAATAAATTGCCACATC8950.10335636813806101No Hit
GTATACTTTTGGCCAGGGGTCCAAGTTGGAGATCAAACGAACTGTGGCTG8700.1004693187487297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG200.00785767450.897795
TTAACGG950.046.9481835
TAGTGAT4800.042.24069270
GTATCAA25400.035.0430761
TAACGGC1300.034.3443836
TAACCGT1350.033.9698153
GGTATCA10700.033.0205421
ATACGTA1253.6379788E-1232.5802311
TACGTAC1253.6379788E-1232.5802312
GTACCCT1400.032.42952347
CGTGCAA1450.031.85849257
CGAACGA450.004145149631.35674353
ACGTACC1307.2759576E-1231.3289513
GTTTAAC1450.030.70715733
TATCAAC28800.030.6400032
CGGTACC1501.8189894E-1230.18144845
AACGGCC1501.8189894E-1229.8069437
ATCAACG29700.029.58873
AACGCAG29850.029.4468146
TCAACGC29950.029.2284284
Produced by FastQC (version 0.11.5)