FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314139
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12090.3848614785174716No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9940.31642043808632486No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9520.3030505604207055No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9120.2903173435963061No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC9050.2880890306520362No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8570.27280917046275693No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTATCTGGGACAGATTTCA8140.25912096237652754No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8050.25625598859103776No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGAGAGA7590.24161278924297844No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA7570.24097612840175844No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7090.22569626821247918No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGAGAGAACTGGTTCTTGGACATGTCTA7020.2234679552682093No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6770.2155096947529597No Hit
GTCTCTGGTGGGTCCATCAGCAGTAGTAACTGGTGGAGTTGGGTCCGCCA6620.21073473844380988No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6520.20755143423771008No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6320.20118482582551037No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6160.1960915390957506No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC6040.1922715740484308No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5920.188451609001111No Hit
GAGTTACATTTCTCGGACTTTCGGCGGAGGGACCAAGGTGGAATCCAAACGAACTGTGGCTGCACCATCTGTCTT5590.17794670512098146No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5520.1757183921767116No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAATGACGCAGTC5440.1731717488118317No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5330.16967011418512187No Hit
GTATTACTGTGCGTCCCGTAATAGACTTGGCTGGCACTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC5290.16839679250268194No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGGATTCCACCTTGGTCC5220.16616847955841205No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5150.16394016661414215No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5060.1610751928286523No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT5060.1610751928286523No Hit
ACTTACTACTGTCAACAGAGTTACATTTCTCGGACTTTCGGCGGAGGGAC4970.15821021904316243No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4920.1566185669401125No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4880.15534524525767254No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4810.15311693231340268No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4700.14961529768669285No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4680.14897863684547286No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4450.1416570371714432No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTCCCAGATACACTGCCA4430.14102037633022324No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4360.13879206338595337No Hit
ATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT4340.13815540254473338No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4300.13688208086229345No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4270.13592708960046349No Hit
GTGCAGCCACAGTTCGTTTGGATTCCACCTTGGTCCCTCCGCCGAAAGTC4210.1340171070768036No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT4210.1340171070768036No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4180.13306211581497362No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA4170.13274378539436363No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC4120.1311521332913137No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTC4090.13019714202948376No Hit
GATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTTCT4080.12987881160887377No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4060.12924215076765383No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4000.1273321682439939No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT3990.1270138378233839No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT3980.12669550740277394No Hit
GTGTATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAA3970.12637717698216394No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3950.12574051614094398No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC3920.12478552487911403No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGGGTCCATCAGCAGTAGTAACTGGT3810.1212838902524042No Hit
AGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG3810.1212838902524042No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAATGACGCAGTCTCCATCCTCC3800.1209655598317942No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3790.12064722941118422No Hit
GTGTATTACTGTGCGTCCCGTAATAGACTTGGCTGGCACTACTACTTTGA3760.11969223814935427No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT3760.11969223814935427No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3650.11619060352264443No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATT3610.11491728184020449No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGTATCTGGGACAGATTTCACT3580.11396229057837454No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3570.11364396015776455No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3500.11141564721349466No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT3470.11046065595166471No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3450.10982399511044473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG3420.10886900384861478No Hit
GTGCCAGATGTGACATCCAAATGACGCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA3370.10727735174556485No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3350.1066406909043449No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC3350.1066406909043449No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC3340.10632236048373492No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT3340.10632236048373492No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG3290.10473070838068499No Hit
ACCTTGGTCCCTCCGCCGAAAGTCCGAGAAATGTAACTCTGTTGACAGTA3210.10218406501580511No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3180.10122907375397516No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC3180.10122907375397516No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT3180.10122907375397516No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3170.10091074333336517No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC3150.10027408249214519No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3150.10027408249214519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)