FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314139
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13770.43834098917994896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9810.31228214261839504No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8700.27694746593068675No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC8360.26612423162994725No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTATCTGGGACAGATTTCA8340.26548757078872726No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8210.2613492753207975No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7650.24352277176663834No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7390.23524618083077872No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7190.22887957241857904No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGAGAGA6840.21773800769722956No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6800.2164646860147896No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA6440.2050047908728302No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC6370.20277647792856027No Hit
GAGTTACATTTCTCGGACTTTCGGCGGAGGGACCAAGGTGGAATCCAAACGAACTGTGGCTGCACCATCTGTCTT6190.19704653035758057No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGAGAGAACTGGTTCTTGGACATGTCTA6010.19131658278660083No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5780.18399498311257118No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG5550.17667338343854153No Hit
GTCTCTGGTGGGTCCATCAGCAGTAGTAACTGGTGGAGTTGGGTCCGCCA5510.1754000617561016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5460.1738084096530517No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5130.16330350577292216No Hit
GTATTACTGTGCGTCCCGTAATAGACTTGGCTGGCACTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC5120.1629851753523122No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5060.1610751928286523No Hit
ACTTACTACTGTCAACAGAGTTACATTTCTCGGACTTTCGGCGGAGGGAC5050.1607568624080423No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5040.1604385319874323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5010.15948354072560236No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGGATTCCACCTTGGTCC4940.15725522778133247No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4900.15598190609889254No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT4820.15343526273401265No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAATGACGCAGTC4750.15120694978974275No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4720.1502519585279128No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4630.14738698474242293No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4630.14738698474242293No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT4480.14261202843327317No Hit
ATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT4330.13783707212412338No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4290.13656375044168345No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4200.1336987766561936No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4180.13306211581497362No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4110.13083380287070373No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT4050.12892382034704383No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4040.12860548992643384No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4020.12796882908521387No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTCCCAGATACACTGCCA3980.12669550740277394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3950.12574051614094398No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGGGTCCATCAGCAGTAGTAACTGGT3940.125422185720334No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTC3930.125103855299724No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3860.1228755423554541No Hit
AGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG3790.12064722941118422No Hit
GATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTTCT3760.11969223814935427No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG3750.11937390772874427No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA3750.11937390772874427No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAATGACGCAGTCTCCATCCTCC3740.11905557730813429No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3610.11491728184020449No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGTATCTGGGACAGATTTCACT3580.11396229057837454No Hit
GTGTATTACTGTGCGTCCCGTAATAGACTTGGCTGGCACTACTACTTTGA3520.11205230805471464No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3510.11173397763410466No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3440.10950566468983475No Hit
GTGTATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAA3430.10918733426922478No Hit
GTGCAGCCACAGTTCGTTTGGATTCCACCTTGGTCCCTCCGCCGAAAGTC3360.10695902132495487No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC3310.10536736922190494No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT3280.10441237796007499No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC3270.10409404753946501No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC3220.1025023954364151No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT3200.10186573459519511No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA3190.10154740417458513No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG3180.10122907375397516No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG3180.10122907375397516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAT100.009632714130.841970
GATTAGA150.002310115169.3276342
GTGGTAT501.8189894E-1161.57991
TCGAGAC200.00680727752.77076357
AGGGCGG200.006989907552.41872450
GTATCAA7850.038.3512461
TATCAAC8950.033.239552
AATGGTA800.003362449132.71047670
TCAACGC9200.032.331164
CAACGCA9250.032.1615145
ATCAACG9250.032.1563953
AACGCAG9400.031.64836
TGTTAGA2700.031.49897870
ACGGGAA553.416049E-431.10095816
GACAGAC450.00481154830.4098281
ACGCAGA9850.030.2024367
AGAGTAC10250.030.02462811
TACTGAT2451.8007995E-1029.37267770
CGCAGAG10250.029.3574148
ACATTAG1401.6370905E-1129.3097577
Produced by FastQC (version 0.11.5)