FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903102
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT45900.5082482377405874No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43880.4858808861014592No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38800.4296303186129584No Hit
GAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGG32330.357988355689612No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31320.34680467987004787No Hit
GTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGTGTGAACT29650.3283128594555211No Hit
GTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTG23710.26253955810085683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23580.2611000750745763No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23320.2582211090220152No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23170.2565601670686146No Hit
GTCTGGGACAGAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATT22210.24593013856685067No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21830.24172241895156912No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT21810.24150096002444904No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20560.22765977707944396No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20410.22599883512604335No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19680.21791558428616037No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18760.20772847363863658No Hit
GTGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATAGA18090.20030959958011388No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17950.1987593870902733No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCT17930.19853792816315322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17340.1920048898131108No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17150.18990103000547004No Hit
GTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17010.1883508175156295No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16230.17971391935794626No Hit
CCACTGGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTC16030.17749933008674545No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15090.16709076051210162No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT15010.16620492480362128No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14830.16421179445954057No Hit
CGTCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTG14090.15601781415609753No Hit
GTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTG13830.15313884810353648No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13790.1526959302492963No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13650.15114571775945573No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12940.14328392584669283No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12490.138301099986491No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12370.1369723464237705No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12220.1353114044703699No Hit
CCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGCACCAGGC11990.13276462680848897No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11550.12789253041184717No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11030.12213459830672505No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11010.12191313937960496No Hit
GTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGCATCCA10910.12080584474400455No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT10850.1201414679626443No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10850.1201414679626443No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC10610.11748396083720333No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACA10570.11704104298296317No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCT10440.11560155995668263No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10440.11560155995668263No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10320.11427280639396213No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10300.11405134746684206No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10160.11250113497700148No Hit
GAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGCATC10120.11205821712276132No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10120.11205821712276132No Hit
CCTCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC9670.1070753912625595No Hit
CCAGTGGCCCTGGTGGATGCACCATAGACGAGGAGCCTGGGAGCCTGGCC9650.10685393233543941No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGGCCTCCGACGTT9640.10674320287187937No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9590.10618955555407915No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9580.10607882609051911No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9500.10519299038203879No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGG9480.10497153145491872No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9410.10419642520999844No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9370.10375350735575825No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC9170.10153891808455744No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9160.1014281886209974No Hit
CACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACACTCACCATCAGCAG9150.10131745915743737No Hit
GGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGG9150.10131745915743737No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9130.10109600023031728No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9120.10098527076675724No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9080.10054235291251709No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9050.10021016452183697No Hit
GTTTATTTCTGTCAGCAGTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA9040.10009943505827693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)