FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903102
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT46430.5141168993092696No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44020.4874310985912998No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41670.4614096746546901No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35260.390432088512704No Hit
GAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGG31000.3432613370361266No Hit
GTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGTGTGAACT30040.3326313085343627No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28260.3129214640206754No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22850.2530168242346933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21580.23895418236256813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21110.23374989757524622No Hit
GTCTGGGACAGAGTTCACACTCACCATCAGCAGCCTGCAGTCTGAAGATT20550.2275490476158839No Hit
GTGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGTGGATGCACCATAGA19820.21946579677600092No Hit
GTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTG19370.2144829709157991No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18910.20938941559203722No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18710.2071748263208364No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18260.20219200046063457No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCT17720.1962126094283924No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17190.1903439478597102No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16970.1879078996613893No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16960.18779717019782927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16410.181707049702027No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16000.17716714169606534No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15500.1716306685180633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15250.16886243192906228No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15010.16620492480362128No Hit
CCACTGGAGAGGTAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTC15000.16609419534006126No Hit
GTCTCCAGCCACCCTGTCTGTGGCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG14670.1624401230425799No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT14270.15801094450017827No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCT13840.15324957756709653No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13830.15313884810353648No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13630.15092425883233568No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12860.14239809013821253No Hit
GTCAGAGTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTG12560.1390762062314113No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12450.13785818213225084No Hit
CGTCTATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTG12160.13464702768900966No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11800.1306607670008482No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11780.1304393080737281No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11060.12246678669740516No Hit
CCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGCACCAGGC11000.12180240991604492No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10840.12003073849908427No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10780.11936636171772402No Hit
GTGTTAGCAGCAACTTGGCCTGGTGCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGCATCCA10700.11848052600924369No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10590.11726250191008325No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10400.11515864210244248No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10220.11316551175836174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10180.11272259390412158No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC9990.1106187340964808No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9740.10785049750747977No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACA9610.10641101448119926No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9550.105746637699839No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGCCAGTT9490.10508226091847875No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC9390.10397496628287833No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA9340.10342131896507814No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9340.10342131896507814No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9310.10308913057439802No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAGGTAGTGATGACGCAGTC9100.10076381183963716No Hit
ATATATTACTGCGCGACCCGCAGGCTAGAGGGACTGGGTTCGGGGAGTTATTATAAGTCCTGGTACTTCGATCT9070.10043162344895705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG200.007088365552.24178344
GGTATCA11600.043.7514081
GTATCAA31750.039.800991
TCGAGTA350.001305168839.68630641
TATCAAC36300.034.779392
CGGCTGA3700.033.57483369
ATCAACG37950.033.3532183
CAACGCA38400.032.8755725
AACGCAG38500.032.703616
TCAACGC38750.032.6628274
ACGCAGA40100.031.3987277
CGCAGAG40650.030.9738988
AGAGTAC41700.030.11404211
GCAGAGT42200.029.999179
TTGTCGG3400.029.53026665
TAGGCGG3350.029.18427847
CGTTCGG13050.028.72835521
ACGAATG904.233276E-628.57497667
CAGAGTA44900.028.11941710
CGGCCAA13500.028.0558825
Produced by FastQC (version 0.11.5)