FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535468
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT49330.9212501960901491No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACTCCAGGAACA20550.3837764348196344No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19010.3550165462735402No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18400.3436246423689184No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14320.26742961297407125No Hit
GTGTATTACTGTGCGAGACGTAGTGGGACCTTCCCGTACTGGTACTTCGA13090.2444590526418012No Hit
GAGTTACATTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13080.2442723001187746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12300.22970560332270087No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11580.2162594216647867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10910.20374700262200543No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10900.20356025009897882No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10710.2000119521614737No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAACTATTTAAATTGGTATCAGCAGAAA10460.19534313908580905No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACTCCAGGAACAACCGATTCTTGGACTTGTCTA10180.19011406844106463No Hit
GATCTATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA9730.18171020490486825No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9670.18058968976670875No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9650.1802161847206556No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9590.17909566958249606No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9580.17890891705946949No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9380.17517386659893774No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9340.17442685650683143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9300.17367984641472506No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT9230.17237257875353895No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAA9120.1703183010002465No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9090.16975804343116677No Hit
GTATTACTGTGCGAGACGTAGTGGGACCTTCCCGTACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA9070.16938453838511358No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9030.16863752829300724No Hit
GTTTCTGGTGGCTCCATCAGCAATAATAATTGGTGGACTTGGGTCCGCCA8760.16359521017128942No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8730.16303495260220965No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8490.1585528920495716No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8360.156125109250226No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8290.15481784158903986No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8020.14977552346732204No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8000.14940201842126888No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7960.14865500832916254No Hit
GAATAGATCAGGAGCTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATT7940.14828150328310935No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC7870.14697423562192324No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7810.14585372048376372No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7720.14417294777652445No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7300.13632934180940784No Hit
ACATTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA7160.13371480648703563No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7150.13352805396400905No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT7150.13352805396400905No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC7120.1329677963949293No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7110.1327810438719027No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG7060.13184728125676978No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6980.1303532610725571No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6970.13016650854953052No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC6940.12960625098045073No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC6780.12661821061202536No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC6740.125871200519919No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA6490.12120238744425438No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6480.12101563492122779No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6450.12045537735214804No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6430.12008187230609485No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG6420.11989511978306829No Hit
GTCCCTCACGTGCGCTGTTTCTGGTGGCTCCATCAGCAATAATAATTGGT6360.11877460464490876No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTACC6230.11634682184556314No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTACCCCCCGGACGTT6190.1155998117534568No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6180.11541305923043021No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6180.11541305923043021No Hit
TCTACAACCCGTCCCTCAAGAGCCGAGTCACCATATCAGTAGACAAGTCCAAGAATCGGTTGTTCCTGGAGTTGA6110.1141057915692441No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6090.11373228652319092No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6060.11317202895411117No Hit
GTCCCTAAGCTCCTGATCTATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT6050.1129852764310846No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5950.11111775120081872No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5920.11055749363173897No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT5870.10962373101660602No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG5780.10794295830936676No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA5770.10775620578634018No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA5760.10756945326331357No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5740.1071959482172604No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAAT5730.10700919569423384No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA5720.10682244317120723No Hit
ATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA5650.10551517551002114No Hit
GGACTGGTGAAGCCTTCGGGGACCCTGTCCCTCACGTGCGCTGTTTCTGG5620.10495491794094139No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC5590.10439466037186161No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG5550.10364765027975528No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT5520.10308739271067552No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5450.10178012504948941No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5430.10140662000343624No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5390.1006596099113299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA100.009421312131.8328170
CTCTTAT2150.056.037061
CGCGTAT252.628058E-454.9330839
AGGGGGG13150.054.63793670
TGTCGTA1900.050.4366338
GTGGTAT800.050.1998671
TATATAG200.00833354650.1436162
GTCGTAT2000.049.783139
CTTGAAA9900.049.2183757
TGCTTGA9750.048.8673255
AAGGGGG10250.048.7380769
GCTTGAA9950.048.413856
CTTATAC9350.048.320731
CCGTCTT9600.048.09115247
CGTATGC9700.048.07374241
TCGTATG9400.048.00382640
CTGCTTG9800.047.73696554
AAAGGGG9350.047.40736868
TCGTCTG305.454592E-447.3769450
ATGCCGT9950.047.35938344
Produced by FastQC (version 0.11.5)