FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535468
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93211.7407202671308089No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACTCCAGGAACA20310.3792943742669963No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19620.3664084501781619No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15420.28797239050699575No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12650.2362419416286314No Hit
GAGTTACATTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12250.22877184070756798No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12160.2270910680003287No Hit
GTGTATTACTGTGCGAGACGTAGTGGGACCTTCCCGTACTGGTACTTCGA11690.21831369941807915No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11030.20598803289832449No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11020.20580128037529788No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAACTATTTAAATTGGTATCAGCAGAAA10220.190861078533171No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACTCCAGGAACAACCGATTCTTGGACTTGTCTA10210.1906743260101444No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10100.18862004825685194No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9910.18507175031934683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9900.18488499779632023No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9710.1813366998588151No Hit
GATCTATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA9430.1761076292140707No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAA9340.17442685650683143No Hit
GTATTACTGTGCGAGACGTAGTGGGACCTTCCCGTACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA9020.16845077576998066No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8960.16733026063182113No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8870.16564948792458187No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8820.16471572530944895No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8520.15911314961865136No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8510.15892639709562476No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8230.15369732645088036No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC8170.15257681131272083No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8080.15089603860548156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8080.15089603860548156No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8040.15014902851337522No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7900.147534493191003No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7890.14734774066797643No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7800.14566696796073716No Hit
GTTTCTGGTGGCTCCATCAGCAATAATAATTGGTGGACTTGGGTCCGCCA7590.14174516497717884No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC7550.1409981548850725No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7540.14081140236204592No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7520.14043789731599274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7480.1396908872238864No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7450.13913062965480663No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7370.13763660947059395No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7290.13614258928638126No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT7200.13446181657914197No Hit
GAATAGATCAGGAGCTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATT7110.1327810438719027No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6920.12923274593439757No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6830.12755197322715828No Hit
ACATTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA6790.12680496313505196No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG6540.1221361500593873No Hit
GTCCCTCACGTGCGCTGTTTCTGGTGGCTCCATCAGCAATAATAATTGGT6470.1208288823982012No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6420.11989511978306829No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA6220.11616006932253654No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC6220.11616006932253654No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTACC6160.11503955418437704No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG6130.11447929661529727No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC6100.11391903904621752No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6000.11205151381595164No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC5980.11167800876989847No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTACCCCCCGGACGTT5970.1114912562468719No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5920.11055749363173897No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA5860.10943697849357945No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5830.10887672092449968No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA5810.10850321587844652No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG5810.10850321587844652No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5780.10794295830936676No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA5760.10756945326331357No Hit
GGACTGGTGAAGCCTTCGGGGACCCTGTCCCTCACGTGCGCTGTTTCTGG5670.10588868055607431No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC5580.10420790784883503No Hit
GTCCCTAAGCTCCTGATCTATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT5560.10383440280278186No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAAT5510.10290064018764894No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5490.10252713514159577No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5480.10234038261856916No Hit
TCTACAACCCGTCCCTCAAGAGCCGAGTCACCATATCAGTAGACAAGTCCAAGAATCGGTTGTTCCTGGAGTTGA5470.1021536300955426No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5450.10178012504948941No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC5420.10121986748040966No Hit
GGGCTGGAGTGGATTGGGGAAATCTATCATGCTGGGAGCAACATCTACAA5420.10121986748040966No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5410.10103311495738307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC150.002682242566.7713212
GTGGTAT1000.043.4581681
AATGGTA1604.5474735E-1142.026470
GATTCAC755.297443E-835.657981
TCTCGCG2850.035.39065670
TGTTAGA5350.035.19220470
GTATCAA11050.032.9755671
TCAGACA1500.032.38807763
TACTGAT7400.031.80376470
TATCAAC12200.029.8281692
ATTCACT902.655761E-729.676142
TAGGAGT450.005425229.676144
ATCAACG12650.029.0310063
TCAACGC12700.028.6538314
CAACGCA12850.028.3193515
TTACAGA1402.8558134E-1028.26950554
AACGCAG13000.027.992596
ACGCAGA13350.027.2587017
CGCAGAG13350.027.2587018
AGAGTAC13500.026.95582811
Produced by FastQC (version 0.11.5)