FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524550
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21370.40739681631874936No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21130.402821466018492No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16810.3204651606138595No Hit
CTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGG14210.27089886569440474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13770.26251072347726623No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12810.2442093222762368No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12760.2432561242970165No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12410.23658373844247452No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11670.2224764083500143No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11360.21656658087884853No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT11320.21580402249547231No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11240.21427890572871983No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11210.21370698694118767No Hit
CAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA11070.21103803259937087No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10850.20684396149080164No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10610.2022686111905443No Hit
GTCTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG10410.19845581927366313No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATCTA9930.18930511867314842No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACGGACTTCACTCTCA9720.18530168716042322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9570.18244209322276236No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9470.1805356972643218No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCC8650.16490325040510914No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8580.16356877323420074No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8550.1629968544466686No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8390.15994662091316367No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8330.15880278333809933No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7910.15079592031264893No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7850.1496520827375846No Hit
GGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7640.14564865122485943No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7620.1452673720331713No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGCCAGG7490.14278905728719857No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7430.14164521971213423No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGC7290.13897626537031743No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7240.13802306739109713No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7070.13478219426174817No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGGAGTTGCTGCTAACAC7040.134210275474216No Hit
CTTCCATACTGCTGACAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTG6990.1332570774949957No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC6980.13306643789915165No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6960.13268515870746353No Hit
ATCTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC6910.13173196072824325No Hit
GCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTT6790.12944428557811458No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6660.12696597083214184No Hit
GTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA6610.12601277285292153No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6450.12296253931941666No Hit
GTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGG6330.12067486416928797No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6310.12029358497759986No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6230.1187684682108474No Hit
GAGTGAAGTCCGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA6220.11857782861500334No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTCTGACGCAGTCTC6170.11762463063578306No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6120.11667143265656277No Hit
CTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGC5960.11362119912305786No Hit
CCTCCACCCTGGTCCCTCCGCCGAAAGTGGAAGAGCTTCCATACTGCTGA5950.1134305595272138No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5940.11323991993136974No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5920.11285864073968163No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5780.11018968639786483No Hit
GGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACGGAC5740.10942712801448862No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5680.10828329043942427No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT5660.10790201124773616No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5650.1077113716518921No Hit
CACCATCACCAGACTGGAGCCTGAAGATTTTGCAATCTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGG5640.10752073205604804No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5630.10733009246020397No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5620.10713945286435991No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5620.10713945286435991No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5610.10694881326851587No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCCCTCCGCCGAAAGTG5600.10675817367267182No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5590.10656753407682776No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5560.10599561528929557No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5550.10580497569345151No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC5510.1050424173100753No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTCTGACGCAGTC5500.10485177771423124No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5420.10332666094747878No Hit
GGTTCAGTGGCAGTGGGTCTGGCACGGACTTCACTCTCACCATCACCAGA5340.10180154418072634No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC5270.10046706700981795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATC359.94342E-441.93903464
CTACGCT2250.039.1374928
GGTATCA6050.039.0863531
GTGTATA350.001493895238.6077461
ACTACGC2300.038.24279427
CGTATGC2000.038.07488641
TATACAC2400.037.9900053
TACGCTG2350.037.50432629
ATGCCGT2050.037.40939344
GAGACTA2350.037.38271324
GTATCAA13900.037.184261
GTATGCC2250.036.9933942
ATCTCGT2050.036.81530437
TCGTATG1900.036.35714740
CGAGACT2450.035.85004823
TCTCCGA2450.035.8363811
TCTGAGT750.002197398335.68342270
CACGAGA2500.035.1330521
ACGCTGC2550.034.60254730
ACGAGAC2550.034.44416822
Produced by FastQC (version 0.11.5)