FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524550
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27630.5267372033171289No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21690.4134972833857592No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19140.3648841864455247No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15570.2968258507291965No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14310.2728052616528453No Hit
CTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGG13990.26670479458583546No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12060.22991135258793252No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12030.22933943380040034No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11240.21427890572871983No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11170.21294442855781143No Hit
GTATGGAAGCTCTTCCACTTTCGGCGGAGGGACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTT11130.21218187017443524No Hit
GTCTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGGGTGTTAGCAG10540.2009341340196359No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10500.20017157563625965No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAATCTA10190.1942617481650939No Hit
CAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA9980.1902583166523687No Hit
ACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACGGACTTCACTCTCA9870.18816128109808408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9770.1862548851396435No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9600.18301401201029455No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9560.1822514536269183No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9190.1751977885806882No Hit
GGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8800.16776284434277No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8500.16204365646744828No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8490.16185301687160425No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8270.157658945763035No Hit
GGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGCCAGG8060.1536555142503098No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8040.15327423505862167No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7840.14946144314174056No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7790.14850824516252026No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCC7680.14641120960823564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7570.14431417405395103No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7470.14240777809551045No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7240.13802306739109713No Hit
CTCCTGCTGGGCCAGTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACC7000.13344771709083977No Hit
GTCAGGGTGTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAGCCTGGC6880.13116004194071107No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6810.12982556476980267No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTCTGACGCAGTCTC6790.12944428557811458No Hit
ATCTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGGCGGAGGGAC6680.12734725002382993No Hit
ATGTATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAAGGAGTTGCTGCTAACAC6560.12505957487370126No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTCTGACGCAGTC6550.12486893527785721No Hit
GTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGCTGGGCCAGTCAGG6530.12448765608616909No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6530.12448765608616909No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6530.12448765608616909No Hit
GCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTACGATACATCGAGCAGGGCCACTGGCATCCCAGACAGGTT6420.12239062053188447No Hit
CTTCCATACTGCTGACAGTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTG6350.12105614336097607No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA6330.12067486416928797No Hit
GTAATAGATTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCCGTGCCAGACCCACTGCCA6260.11934038699837957No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6230.1187684682108474No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6140.11705271184825088No Hit
GAGTGAAGTCCGTGCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA6130.11686207225240683No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6100.11629015346487466No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5900.11247736154799351No Hit
GGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACGGAC5810.110761605185397No Hit
GACCAGGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT5710.10885520922695643No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5670.10809265084358022No Hit
CACCATCACCAGACTGGAGCCTGAAGATTTTGCAATCTATTACTGTCAGCAGTATGGAAGCTCTTCCACTTTCGG5540.10561433609760748No Hit
CTCCAGGCATCCTGTCTTTGTCTGCAGGGGAGAGAGCCACCCTCTCCTGC5460.10408921933085502No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC5370.1023734629682585No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCCTGGTCCCTCCGCCGAAAGTG5300.1010389857973501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)