FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965436
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40490.41939600346372No Hit
GACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAAATAAGTGCCA37980.39339738729444523No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37110.3843859147576846No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36930.3825214721638721No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33610.34813286432244084No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32490.3365318881831629No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31340.32462017161158274No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGAACTGCCAAAG30010.31084401244619014No Hit
GTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG29740.3080473485554713No Hit
GATTACTACTGTCAATCAGCAGACACCAGTGGCACTTATTTTGTCTTCGG29710.3077366081231692No Hit
GGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC28960.299968097315617No Hit
GCAATATACTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCC28520.29541057097518636No Hit
GCCTTGGGCTGACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAA28130.2913709453552592No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26780.27738762590166516No Hit
GTAGTAATCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA26250.27189787826432826No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25880.2680654129326025No Hit
CTCTCTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGG24600.25480715448771335No Hit
GTATATTGCTTTGGCAGTTCATCTCCAGAGCAGGTGATCCTGGCCGTCTG23650.24496704079814716No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22990.2381307512875012No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22940.2376128505669977No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC22030.22818705745383433No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21670.2244581722662093No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAAATAAGTGC21230.21990064592577863No Hit
GTAATATATTGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCAGATGCA21180.21938274520527512No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21090.2184505239083689No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19880.2059173264721846No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18470.1913125261539864No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17510.18136883232031953No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCA17410.18033303087931254No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17360.1798151301588091No Hit
ATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATTGCTTT17000.176086244971184No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16770.173703901656868No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGT16620.17215019949535754No Hit
CCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGGGATCCCT16450.17038933704564568No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15260.15806329989766282No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15130.15671675802435375No Hit
AGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGAACTGC15030.1556809565833468No Hit
GTCATTATATATCACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACC14840.15371293384543355No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14800.15329861326903077No Hit
GGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGG14390.14905182736090222No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14320.14832676635219733No Hit
GGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCT14050.14553010246147854No Hit
ATATAATGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT13630.1411797364092493No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13440.13921171367133606No Hit
CTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGT13410.13890097323903397No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13340.1381759122303291No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13030.1349649277632075No Hit
CACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATT13030.1349649277632075No Hit
GTCCAGGCAGAAGACGAGGCTGATTACTACTGTCAATCAGCAGACACCAGTGGCACTTATTTTGTCTTCGGAC12640.13092530214328035No Hit
GCTCTGGAGATGAACTGCCAAAGCAATATACTTATTGGTACCAGCAGAAG12610.13061456171097824No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12610.13061456171097824No Hit
GGATCACCTGCTCTGGAGATGAACTGCCAAAGCAATATACTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC12540.12988950070227337No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12510.12957876026997128No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12450.1289572794053671No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12430.12875011911716572No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12420.12864653897306502No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12250.12688567652335317No Hit
CTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAG12210.1264713559469504No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12170.1260570353705476No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT12170.1260570353705476No Hit
ATATATTACTGTGCGAGAGTCTCCTTAGGTTTTGACCTGTTCTACTTTGA12020.12450333320903717No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11910.12336395162392949No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT11900.12326037147982881No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11860.12284605090342601No Hit
CCCTGGTCCCAGGTCCGAAGACAAAATAAGTGCCACTGGTGTCTGCTGATTGACAGTAGTAATCAGCCTCGTCTT11800.12222457003882184No Hit
GTCCTGGGGACACTGACACGGAGGGTGCCTGTGTCAGCTCATGTGAGGCC11750.12170666931831835No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTACTACTGTCAATCAGCA11580.11994580686860651No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11540.11953148629220373No Hit
GGTGTCTGCTGATTGACAGTAGTAATCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGT11510.11922074585990164No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTAC11510.11922074585990164No Hit
ACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAAATAAGTGCCAC11340.1174598834101898No Hit
GTACGGGGCTGTGGGTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTC11070.11466321951947099No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10970.11362741807846403No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGATTACTACTGTCAATCAGCAGAC10960.11352383793436334No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT10900.11290235706975915No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG10810.11197013577285289No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT10750.1113486549082487No Hit
CACATGAGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACG10720.11103791447594662No Hit
AGCCAGGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCC10510.108862731449832No Hit
CCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAT10500.1087591513057313No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCA10470.10844841087342921No Hit
GCAGAAGCCAGGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATT10460.1083448307293285No Hit
GCAGACACCAGTGGCACTTATTTTGTCTTCGGACCTGGGACCAGGGTCCTCGTCCTAGGTCAGCCCAAGGCCAAC10390.10761976972062365No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10360.10730902928832153No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10330.10699828885601945No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT10300.10668754842371737No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10300.10668754842371737No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACG10210.1057553271268111No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10200.10565174698271039No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT10180.10544458669450901No Hit
CCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATTGC10100.10461594554170345No Hit
GGAGATGAACTGCCAAAGCAATATACTTATTGGTACCAGCAGAAGCCAGG10040.10399446467709926No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGAACTGCCAAAGCAATA9970.10326940366839439No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA9920.1027515029478909No Hit
GGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTG9900.1025443426596895No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9830.10181928165098462No Hit
GCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGTCA9680.10026557948947419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2100.078.6181670
GTACCTC200.007647783551.248033
GGTATCA6750.049.1172331
ATGCCGT2200.044.17880244
CGTATGC2000.043.23971641
GCCGTTA405.299121E-542.70448315
CTTATAC2350.042.1789781
TCGTATG2050.042.12985240
TCTCCGA2350.040.70765711
CGAGACA2450.040.44481323
TCTCGTA1800.040.21576738
TATACAC2750.039.7560463
ACGAGAC2500.039.63386522
ATCTCCG2500.039.63181310
GTATGCC2500.038.78957442
GGTACGT400.001555816638.29272568
CCGTTAA451.0606018E-437.96150616
GTATCAA17700.037.65541
ATCTCGT1850.037.22472837
CCCACGA2600.036.79536419
Produced by FastQC (version 0.11.5)