FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965436
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37170.3850073956222888No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC36750.38065702957005954No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34990.36242692420833694No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32550.337153369047767No Hit
GACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAAATAAGTGCCA32080.33228510227503427No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29950.3102225315815859No Hit
GATTACTACTGTCAATCAGCAGACACCAGTGGCACTTATTTTGTCTTCGG29490.3054578449529539No Hit
GTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG29250.3029719214945372No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGAACTGCCAAAG28050.2905423042024536No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27940.28940292261734596No Hit
GCCTTGGGCTGACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAA27240.28215231253029716No Hit
GCAATATACTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCC26980.27945922878367907No Hit
GGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC26920.2788377479190749No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24820.25708591765792865No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23980.24838518555347014No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG23910.24766012454476527No Hit
CTCTCTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGG23560.24403481950124087No Hit
GTATATTGCTTTGGCAGTTCATCTCCAGAGCAGGTGATCCTGGCCGTCTG23110.23937371301670954No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22960.23782001085519913No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22440.2324338433619629No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21600.2237331112575044No Hit
GTAGTAATCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA21120.21876126434067097No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAAATAAGTGC20330.21057843295671594No Hit
GTAATATATTGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCAGATGCA19730.20436362431067415No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC19570.20270634200506302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19290.19980609797024348No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18530.19193400701859056No Hit
CCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGGGATCCCT18390.19048388500118082No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17840.1847869770756425No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17710.18344043520233344No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGT17640.18271537419362857No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16950.17556834425068052No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCA16780.17380748180096867No Hit
ATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATTGCTTT15780.16344946739089902No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15640.16199934537348928No Hit
AGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGAACTGC15270.15816688004176352No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14220.1472909649111904No Hit
GTCATTATATATCACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACC14090.14594442303788133No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14060.14563368260557924No Hit
ATATAATGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT13880.1437692400117667No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13870.143665659867666No Hit
CTGCACAGGCTCTGAGGCCTCACATGAGCTGACACAGGCACCCTCCGTGT13520.14004035482414165No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13040.1350685079073082No Hit
GGCACCCTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCT12940.13403270646630125No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12860.13320406531349566No Hit
GGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGG12860.13320406531349566No Hit
GGATCACCTGCTCTGGAGATGAACTGCCAAAGCAATATACTTATTGGTACCAGCAGAAGCCAGGCCAGGCCC12770.1322718440165894No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12760.1321682638724887No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12710.13165036315198522No Hit
CACCAATACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGTATATT12620.13071814185507893No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12610.13061456171097824No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12510.12957876026997128No Hit
CTCCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAG12440.1288536992612664No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12200.1263677758028497No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTACTACTGTCAATCAGCA12140.12574629493824552No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12040.12471049349723855No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11940.1236746920562316No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11920.1234675317680302No Hit
GTCCAGGCAGAAGACGAGGCTGATTACTACTGTCAATCAGCAGACACCAGTGGCACTTATTTTGTCTTCGGAC11760.12181024946241906No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11650.1206708678773114No Hit
CCCTGGTCCCAGGTCCGAAGACAAAATAAGTGCCACTGGTGTCTGCTGATTGACAGTAGTAATCAGCCTCGTCTT11550.11963506643630442No Hit
GCTCTGGAGATGAACTGCCAAAGCAATATACTTATTGGTACCAGCAGAAG11460.11870284513939816No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11410.11818494441889467No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGATTACTACTGTCAATCAGCAGAC11400.11808136427479397No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11370.11777062384249189No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11240.11642408196918283No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11220.11621692168098145No Hit
ATATATTACTGTGCGAGAGTCTCCTTAGGTTTTGACCTGTTCTACTTTGA11130.11528470038407518No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10840.11228087620515498No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGATTAC10810.11197013577285289No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10810.11197013577285289No Hit
GGTGTCTGCTGATTGACAGTAGTAATCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGT10700.11083075418774523No Hit
AGCCAGGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCC10680.11062359389954383No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT10640.11020927332314105No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC10630.11010569317904036No Hit
GTACGGGGCTGTGGGTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTC10630.11010569317904036No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC10430.10803409029702643No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT10350.10720544914422085No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCA10330.10699828885601945No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG10310.10679112856781807No Hit
GCAGACACCAGTGGCACTTATTTTGTCTTCGGACCTGGGACCAGGGTCCTCGTCCTAGGTCAGCCCAAGGCCAAC10270.10637680799141529No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10130.10492668597400553No Hit
CCGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAT10120.10482310582990483No Hit
ACCTAGGACGAGGACCCTGGTCCCAGGTCCGAAGACAAAATAAGTGCCAC10100.10461594554170345No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCTCTCA10080.10440878525350204No Hit
GTCCTGGGGACACTGACACGGAGGGTGCCTGTGTCAGCTCATGTGAGGCC10040.10399446467709926No Hit
GCAGAAGCCAGGCCAGGCCCCTGTATTGGTGATATATAATGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATT10030.10389088453299855No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGAACTGCCAAAGCAATA9900.1025443426596895No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA9790.10140496107458184No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC9730.10078348020997768No Hit
CACATGAGCTGACACAGGCACCCTCCGTGTCAGTGTCCCCAGGACAGACG9700.10047273977767557No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9670.1001619993453735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)