FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141432
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAGCTCCGACACTAAGAACTACTTAGCTTGGTTCCAGCAGAA91150.7985583022028471No Hit
GTATAAGACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA59430.5206617652212309No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38780.3397486665872343No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38190.33457972091197724No Hit
GCTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT34100.2987475381801106No Hit
ATATTATAGCAGTCCGATCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT31890.27938589421008No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTAGTGTCGGAGCTGTATAAGACACT31480.27579391501202No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGCAATCCGGGGTCCCT31030.2718514988190273No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29940.26230209070711175No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27500.24092543401621822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25940.22725839121384367No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25420.22270271027971883No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24030.21052502470580814No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23820.20868523048241158No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22490.19703320040089992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22010.19282795646170775No Hit
GTAATACATAGCCGTGTCTTCGTCTCTCAGATTGTTCATTTGCAGAAACA21680.18993685125351314No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21170.18546877956812147No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20770.18196440961879462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20700.18135114487766243No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20510.1796865691517322No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20420.17889808591313366No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19120.16750888357782154No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18750.16426734137469423No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18010.15778425696843965No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAACTGGG17560.153841840775447No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTATA16740.14665788237932703No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16460.14420482341479826No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16420.1438543864198656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16390.14359155867366608No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15690.13745891126234414No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15460.13544389854148123No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15380.13474302455161585No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15360.13456780605414953No Hit
ATGTATTACTGTACAAGGTCGGGTGGGAGCGACTCCCGATTTGACCACTGGGGCCAGGGAACCCTGGTCACCGT15260.13369171356681783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15140.13264040258201978No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14750.12922364188142615No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTATACAGCTCCGA14650.12834754939409443No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14500.12703341066309687No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGCAA14310.12536883493716666No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14100.12352904071377008No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAA14090.1234414314650369No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14080.12335382221630373No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCAGTTACTGGATGCACTGGGTCCGCCTAGCTCCAGGGAAGGGG14060.12317860371883738No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14030.12291577597263789No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14020.12282816672390472No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG13730.12028749851064277No Hit
GATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGCAGT13710.12011228001317642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13670.11976184301824375No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAACTGGGTTTTC13480.11809726729231351No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA13430.11765922104864768No Hit
ACCTTGTACAGTAATACATAGCCGTGTCTTCGTCTCTCAGATTGTTCATTTGCAGAAACAGTGTGTTCTTGGCGT13280.1163450823176501No Hit
GTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTAGTGTCGGAGCTG13130.11503094358665256No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13030.11415485109932084No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12930.11327875861198915No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTCTTATACAGCTCCGACA12600.11038765340379453No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12360.10828503143419846No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12250.10732132969813357No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12200.10688328345446774No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC12130.10627001871333552No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12080.10583197246966967No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11670.10223999327160971No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11660.10215238402287653No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11640.1019771655254102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAACG200.00784925550.9126363
CGTATGC3700.048.4964741
ATCTCGT2650.047.8473137
TCGTATG3600.047.815840
CACGAGA3800.046.46290221
GGTATCA8150.046.247691
ATGCCGT3850.045.91108744
GTGGTAT2050.044.7233731
GTATGCC3950.044.6190542
GATCTCG2850.044.4424336
CGATCTC2950.044.0399135
GAGCGAT3800.043.9769932
CGAGACA4100.043.89708723
TGGTATC2100.043.6394042
ACGAGAC4150.043.36250322
TGCCGTC4000.042.53641545
GCGATCT3000.042.11981634
CCGTCTT4150.041.12427547
GCCGTCT4150.041.06520546
CGAGCCC4300.041.0476515
Produced by FastQC (version 0.11.5)