FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160775
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT47130.4060218388576597No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45890.39533932071245503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41430.35691671512567036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41040.3535568908703237No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38280.3297796730632551No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32200.2774008744157998No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30250.2606017531390666No Hit
CTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGGCTGCACCA30230.2604294544593052No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30160.25982640908014043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29570.2547435980271801No Hit
GTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC29560.25465744868729945No Hit
TTATAATACTTATTCTCATGTGTACACTTTTGGCCAGGGGACCGAGCTGG28850.24854084555577094No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27360.23570459391354914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26910.23182787361891838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25140.2165794404600375No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24880.2143395576231397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24490.21097973336779308No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24250.20891214921065665No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24130.20787835713208846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24040.2071030130731623No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23140.19934957248390084No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21210.182722749886929No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA20940.18039671771015053No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20540.17695074411492323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19600.168852706166139No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19240.1657513299304344No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19120.1647175378518662No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTA18870.16256380435484913No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18230.1570502466024854No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17930.1544657664060649No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC17910.15429346772630356No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17820.15351812366737738No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17820.15351812366737738No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAA17810.15343197432749672No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17650.1520535848894058No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17640.1519674355495251No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17030.14671232581680343No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC16940.14593698175787728No Hit
GTATTACTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16760.14438629364002498No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16580.1428356055221727No Hit
GGCCAAAAGTGTACACATGAGAATAAGTATTATAATGTTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGG16080.13852813852813853No Hit
CTAGTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGAAAGGGTGGAAGGAGA15720.13542676229243394No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTA15590.13430682087398504No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15150.130516249919235No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14980.12905171114126338No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14950.12879326312162132No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14820.12767332170317247No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14730.1268979776442463No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14630.12603648424543945No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14550.125347289526394No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14500.12491654282699059No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14420.12422734810794511No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14120.12164286791152464No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAACTAGTAATACTCTGA13950.12017832913355303No Hit
CCTTCCACCCTTTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG13800.11888608903534276No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTCGGTCC13640.11750769959725184No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13600.1171631022377291No Hit
TGTGTACACTTTTGGCCAGGGGACCGAGCTGGAGATCAAACGAACTGTGG13490.11621545949904159No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13390.11535396610023475No Hit
GTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13370.1151816674204734No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG13340.11492321940083133No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTCGGTCCCCTGGCCAAAAGTG13310.11466477138118929No Hit
GTCTCCTTCCACCCTTTCTGCATCTGTGGGAGACAGAGTCACCATCACTT12970.11173569382524606No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12830.1105296030669165No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12500.10768667485085395No Hit
GTAATAGATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG12450.10725592815145053No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12300.10596368805324029No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11980.10320690917705842No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11950.10294846115741638No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11790.10157007171932544No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11670.10053627964075726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25450.040.720941
ATCAACG28850.035.4279373
TCAACGC28950.035.1886564
CAACGCA29200.034.890395
TATCAAC29400.034.766672
AACGCAG29750.034.1345256
TTTAAGT2153.45608E-1133.95413270
ACGCAGA30550.033.351467
AGAGTAC32050.032.52986511
CGCAGAG31550.032.4016538
GTGGTAT3350.032.3511241
GCAGAGT32400.031.9695139
TTAACGG953.4405275E-728.81694435
CGGGTTT855.1000025E-627.9005717
TGTTAGA17650.027.44837270
ACGGGGT5950.026.18898416
GACAGGT3000.025.9651451
TAACCGT1109.279174E-725.7231453
GAGTACG31350.025.69687312
AGTACGG31400.025.22693813
Produced by FastQC (version 0.11.5)