FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991870
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG40160.4048917700908385No Hit
GATTATTACTGTGCAGCGTGGGATGACAGCCTGAATGGTCCGGTCTTCGG40090.40418603244376783No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA35750.3604302983253854No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29720.29963604101343927No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCTGGGACCC28780.2901589926099186No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28290.28521882908042384No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27830.28058112454253076No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26960.2718098137860808No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25570.2577958805085344No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25520.2572917821891982No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24240.2443868652141914No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCT23980.2417655539536431No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23580.2377327673989535No Hit
GTATTACTCCCGATGTTGGAGCTGCTTCCAGAACAAGACATGGTGACCCT22930.23117948924758283No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGGTCCGAAGACCGGACCATTCAGGCTGTCATCCCACG22470.22654178470968978No Hit
GTCTATTACTGTGCGAAAGGGACCTACGATTTTTGGAGTTCTTACCCACT21130.21303194975147954No Hit
GAGTAATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGAC19190.19347293496123485No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18310.18460080454091765No Hit
GTGCTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18080.18228195227197114No Hit
ACCCAGGACGGTGACCTTGGTCCCAGGTCCGAAGACCGGACCATTCAGGC17740.17885408370048494No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17400.17542621512899875No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16490.16625162571707985No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16380.1651426094145402No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16290.16423523243973503No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15630.15758113462449716No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15270.15395162672527649No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAGCACGCTGTATCTG15230.15354834806980752No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCAC15190.15314506941433859No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14830.1495155615151179No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14470.14588605361589724No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13870.1398368737838628No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13810.13923195580065936No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13300.1340901529434301No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTC13290.13398933327956286No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13270.13378769395182838No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACAGCCTGAATGGTCCGGTCTTCGGACCTGGGAC12990.13096474336354563No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12960.1306622843719439No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC12770.12874671075846633No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAACAACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGCAGGGGG12710.12814179277526289No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12470.12572212084244913No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCACAGTATTACTC12380.12481474386764393No Hit
GTATAGTGGGTAAGAACTCCAAAAATCGTAGGTCCCTTTCGCACAGTAAT12310.12410900622057326No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT12130.12229425227096294No Hit
CCCCTGAGGGCCGCTGATTATTAATATGGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCA12030.12128605563229052No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11870.11967294101041467No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11770.11866474437174226No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11760.11856392470787502No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11620.11715244941373365No Hit
GTATGTGCTAGTACCACTTCCAATAACAGCTGAGACCCACTCCAGCCCCCTGCCTGGAGCCTGGCGGACCCAGGT11590.11684999042213193No Hit
GACCCAGGACGGTGACCTTGGTCCCAGGTCCGAAGACCGGACCATTCAGG11380.11473277748091988No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11360.1145311381531854No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11280.11372458084224746No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11250.11342212185064575No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATGTCTTGTTCTGG11100.11190982689263715No Hit
GTCTTGTTCTGGAAGCAGCTCCAACATCGGGAGTAATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCC11080.11170818756490267No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10940.11029671227076128No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10880.10969179428755783No Hit
CTATACTGGGGCCTGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGC10830.10918769596822164No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10810.10898605664048716No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG10710.10797786000181475No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10630.10717130269087684No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10480.10565900773286821No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG10470.10555818806900097No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10330.10414671277485961No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10130.10213031949751479No Hit
TTACTGTGCAGCGTGGGATGACAGCCTGAATGGTCCGGTCTTCGGACCTG10100.10182786050591307No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT10080.10162622117817859No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9990.10071884420337343No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9970.10051720487563895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)