FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991870
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCGTGGGATGACAGCCTGAATGGTCCGGTCTTCGG38180.38492947664512484No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA31580.31838849849274603No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCTGGGACCC31010.31264177765231327No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28090.283202435803079No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27180.2740278463911601No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25890.26102210975228607No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25130.25335981529837576No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23980.2417655539536431No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCACCCTCAGCGTCT23930.24126145563430693No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23290.2348089971468035No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23270.23460735781906905No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22510.22694506336515874No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG21970.22150080151632773No Hit
GTATTACTCCCGATGTTGGAGCTGCTTCCAGAACAAGACATGGTGACCCT21430.21605653966749674No Hit
GAGTAATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGAC20780.2095032615161261No Hit
GTCTATTACTGTGCGAAAGGGACCTACGATTTTTGGAGTTCTTACCCACT20060.2022442457176848No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGGTCCGAAGACCGGACCATTCAGGCTGTCATCCCACG19870.20032867210420718No Hit
GTGCTGACTCAGTCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19210.19367457428896934No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16870.17008277294403498No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16850.16988113361630053No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16750.16887293697762812No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16680.16816719933055743No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16500.1663524453809471No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAGCACGCTGTATCTG15230.15354834806980752No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15160.15284261042273686No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15150.15274179075886962No Hit
ACCCAGGACGGTGACCTTGGTCCCAGGTCCGAAGACCGGACCATTCAGGC15140.15264097109500238No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGTCAC14810.14931392218738343No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13900.1401393327754645No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13780.13892949680905764No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACAGCCTGAATGGTCCGGTCTTCGGACCTGGGAC13450.1356024479014387No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13360.13469507092663352No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13040.13146884168288184No Hit
GTCTTGTTCTGGAAGCAGCTCCAACATCGGGAGTAATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCC13030.1313680220190146No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAACAACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGCAGGGGG12930.1303598253803422No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12780.12884753042233357No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12680.12783933378366116No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12650.12753687479205944No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12100.12199179327936122No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTC12050.121487694960025No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATGTCTTGTTCTGG12000.12098359664068879No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11940.12037867865748535No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11850.11947130168268019No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11850.11947130168268019No Hit
CCCCTGAGGGCCGCTGATTATTAATATGGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCA11840.11937048201881295No Hit
GTATAGTGGGTAAGAACTCCAAAAATCGTAGGTCCCTTTCGCACAGTAAT11800.11896720336334399No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTCACAGTATTACTC11750.1184631050440078No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11680.11775736739693708No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT11630.11725326907760089No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11360.1145311381531854No Hit
GTATGTGCTAGTACCACTTCCAATAACAGCTGAGACCCACTCCAGCCCCCTGCCTGGAGCCTGGCGGACCCAGGT11300.11392622016998194No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11160.11251474487584058No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10800.10888523697661992No Hit
GCCCCCAGACTCCTCATCCATATTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT10380.10465081109419581No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA10320.10404589311099237No Hit
CTATACTGGGGCCTGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGC10300.10384425378325789No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10250.1033401554639217No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10190.10273523748071822No Hit
GGGTCACCATGTCTTGTTCTGGAAGCAGCTCCAACATCGGGAGTAATACT10150.10233195882524927No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10130.10213031949751479No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9960.10041638521177171No Hit
TTACTGTGCAGCGTGGGATGACAGCCTGAATGGTCCGGTCTTCGGACCTG9950.10031556554790447No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC9920.10001310655630274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)