FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754555
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27510.36458574921642556No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26410.35000762038552524No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25530.33834511732080497No Hit
GTAATAAACGGCCGTGTCGTCGACTTTCAGGCTGTTCATTTGCAGGTACA24640.32655008581216743No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22890.3033576081266442No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22300.2955384299355249No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21450.2842735122025565No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20990.2781772037823618No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG20990.2781772037823618No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19350.2564425389799286No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19100.25312932788199666No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGTCTCT18550.2458402634665465No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG18530.24557520657871196No Hit
GTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGC18310.2426595808125319No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17130.22702122443029335No Hit
CCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT16910.2241055986641133No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16890.2238405417762787No Hit
GCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCT16750.2219851435614369No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGAATGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC16170.21429849381423485No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATG16130.2137683800385658No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16090.2132382662628967No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGTTGTGC15930.21111781116022027No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15890.21058769738455116No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15850.21005758360888205No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC15330.20316610452518372No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15150.20078059253467279No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT14910.19759990988065815No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14700.19481681255839536No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14520.19243130056788438No Hit
CCTGCACTCTGAGCGGTGGGCACAACAGCTACGCCATCGCATGGCATCAG14350.19017831702129068No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13950.18487717926459968No Hit
GCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGTCGACTTTCA13940.1847446508206824No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG13900.18421453704501328No Hit
GTCGTATAGCCACTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGT13840.18341936638150963No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTCATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC13790.18275672416192323No Hit
GTTTATTACTGTGCGAGGACCGGGTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGT13360.1770580010734804No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13280.1759977735221422No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13120.17387731841946577No Hit
CACCTGCACTCTGAGCGGTGGGCACAACAGCTACGCCATCGCATGGCATC12990.17215444864854118No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12530.16605814022834653No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11850.15704620604197175No Hit
CAGTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC11680.15479322249537808No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11620.1539980518318744No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACA11290.14962461318260434No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGTCAGAC11290.14962461318260434No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAG11120.14737162963601064No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG11100.14710657274817607No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC10930.1448535892015824No Hit
ACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG10810.1432632478745751No Hit
GGGTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGC10760.1426006056549887No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGT10530.13955245144489137No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10510.1392873945570568No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10420.13809463856180132No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10400.13782958167396678No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10390.1376970532300495No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10310.1366368256787113No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10190.135046484351704No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTA10040.13305855769294483No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9820.1301429319267648No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9800.12987787503893022No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9780.12961281815109568No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTATTGGAGT9770.1294802897071784No Hit
CTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGTCGACTTT9760.1293477612632611No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9590.12709477771666744No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9570.1268297208288329No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9560.1266971923849156No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT9540.12643213549708107No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9410.12470926572615648No Hit
GTGTGAGGTGCAGCTATTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC9390.12444420883832193No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9250.12258881062348008No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9230.12232375373564552No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9060.12007077018905182No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCAGTCGTATAGCCACTG8950.11861295730596179No Hit
GTATAGCAGTGGCTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCC8950.11861295730596179No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8940.1184804288620445No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8940.1184804288620445No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8920.11821537197420998No Hit
TCCTCACCCTCCTCCTCCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT8910.11808284353029268No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8900.11795031508637542No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT8800.11662503064720266No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8790.11649250220328539No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTACACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC8790.11649250220328539No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC8670.11490216087627807No Hit
GGGCACAACAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAGGG8610.11410699021277443No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8510.11278170577360166No Hit
GGGCTGGAGTGGGTCTCAGCTCTTAGTGGTGCTGCTACTGGCAGATACTA8480.11238412044184984No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8480.11238412044184984No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8430.11172147822226346No Hit
GAGTGCAGGTGAGCTTGACCGAGGCTCCCAGGGAGGCAGAGGCAGAGGGC8300.10999860845133888No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8180.10840826712433156No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8160.108143210236497No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8140.10787815334866245No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8140.10787815334866245No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8120.10761309646082791No Hit
GGGTTCCCTGGCCCCAGTCAGTCGTATAGCCACTGCTATACCCGGTCCTCGCACAGTAATAAACGGCCGTGTCGT8100.10734803957299335No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8050.10668539735340697No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8030.10642034046557242No Hit
CTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTACCTGCAAATGAA8020.10628781202165515No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT8010.10615528357773786No Hit
ATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGC7940.10522758447031695No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGT7860.10416735691897874No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC7770.10297460092372325No Hit
CTATACGACTGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCAT7730.10244448714805415No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACT7690.10191437337238504No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7620.1009866742649641No Hit
ATACTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATT7590.10058908893321229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2500.093.9121670
TATTAGA252.6779878E-454.727352
CGAAATT200.00694352152.51439753
TTATTCG200.007340970451.78039641
GTGTTAC200.007594585451.3375051
ATAGTGT200.00761052551.3102878
CCCTATA200.00761251951.306892
GATAGAG200.00761251951.306893
GCGATCT2000.049.78801334
ATCTCGT2050.048.6935237
CCCACGA2750.048.5083319
GCCGTCT2750.047.92830346
TCGTATG2600.047.7525840
GGTATCA6600.047.707581
TCTCGTA2100.047.5595238
CGATCTC2100.047.4455635
CGTATGC2850.047.23825541
AAAGGGG3000.047.16081668
CACGAGA2850.046.80938721
TCTCCGA2800.046.4235911
Produced by FastQC (version 0.11.5)