FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090058
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC40380.37043900416308123No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32700.299984037546626No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28910.26521524542730757No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26850.24631716844424795No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC22770.20888796742925606No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20880.19154944048848777No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20080.18421038146594035No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19080.17503655768775608No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA18990.17421091354771948No Hit
GGATGAGGCTGACTATTACTGTAATTCCCGGCACAGTAGTGATTATTATC18670.1712752899387005No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCAG18100.16604621038513548No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17400.15962453374040647No Hit
GGGACAGACAGTCCGGATCACATGCCAAGGAGACAGCCTCAGAACCAATT17360.1592575807892791No Hit
GTAAAGGGGCCTGTCCTGGCTTCTTTTGGTACCAGGTTGCATAATTGGTT17340.1590741043137154No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17030.1562302189424783No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTGCT17020.15613848070469644No Hit
GTAGTGATTATTATCTGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTG16780.1539367629979322No Hit
GTCCGGATCACATGCCAAGGAGACAGCCTCAGAACCAATTATGCAACCTGGTACCAAAAGAAGCCAGGACAGGCC16770.15384502476015038No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16630.15256068943120457No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT16340.14990028053553114No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16270.14925811287105822No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16240.1489828981577127No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGA16210.1487076834443672No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16120.14788203930433058No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14890.13659823605716392No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14690.13476347130152708No Hit
GCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAACATGTGTCCCCTGAACTGGAGCCAGAGAATCGGT14580.13375435068592678No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14520.13320392125923575No Hit
GTCTGTGGCCTTGGGACAGACAGTCCGGATCACATGCCAAGGAGACAGCCTCAGAACCAATTATGCAACCTGGTA14190.13017655941243494No Hit
CTATTACTGTAATTCCCGGCACAGTAGTGATTATTATCTGGTGTTCGGCG13960.12806657994345255No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13810.1266905063767249No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13640.12513095633443358No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13560.12439705043217884No Hit
AGCCAGGACAGGCCCCTTTACTTGTCATTTATGCTAAGGACAAACGGCCC13160.12072752092090512No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCCGGATCAC13100.12017709149421407No Hit
AATCACTACTGTGCCGGGAATTACAGTAATAGTCAGCCTCATCCTCCGCC13090.12008535325643223No Hit
CTTCTGAACTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA12950.11880101792748643No Hit
GGACAAACGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGTTCAGGGGACACATGTTCCTTGACCATCA12870.1180671120252317No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12660.116140609031813No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12530.11494801194064903No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12480.11448932075173981No Hit
GTCTCTGGTGACTCCCTCACAAATGACTATTGGAGTTGGATCCGGCAGCC12470.11439758251395797No Hit
CAATAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGAACTGATTCTTGGTCGTGTCTA12360.1133884618983577No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT12010.1101776235759932No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11950.10962719414930215No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11930.10944371767373846No Hit
CTACTGTGCCGGGAATTACAGTAATAGTCAGCCTCATCCTCCGCCTGAGC11900.10916850296039293No Hit
GTCCTGGACCTCCTGTGCCAGAACATGAAACATCAGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11840.10861807353370187No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11650.10687504701584687No Hit
GTCCTGGCTTCTTTTGGTACCAGGTTGCATAATTGGTTCTGAGGCTGTCTCCTTGGCATGTGATCCGGACTGT11370.10430637635795525No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11240.10311377926679131No Hit
TCACTGGGGCTCAGGCGGAGGATGAGGCTGACTATTACTGTAATTCCCGG11190.1026550880778821No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAA11010.10100379979780891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4050.061.6516970
GTGGTAT2900.049.6089361
TGGTATC3100.045.2763822
GGTATCA11100.044.7459371
ACTAGCG3500.044.0729527
CTAGCGC3550.043.47017328
CGTATGC3600.043.25398341
TCGTATG3600.043.197940
TAGCGCT3650.042.2889229
ATCTCGT3350.041.16644737
TCTCGTA3500.040.42270738
CACGAGA3900.040.3815221
GACTAGC3850.040.0552826
CGCTCAT3650.039.51688432
CTTATAC3950.039.0233041
ACGAGAC3950.039.005422
ATCTCCG4000.038.51606410
GCGCTCA4050.038.13502531
CGAGACT4200.037.50400523
GTATCAA26150.037.3320431
Produced by FastQC (version 0.11.5)