FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090058
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC36900.33851409741499994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31410.2881498048727682No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30140.27649904867447417No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29010.266132627805126No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25010.22943733269238883No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCAG23820.21852048239634955No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC21140.19393463467081568No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19290.17696306068117476No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18900.1733852694076829No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA18330.16815618985411784No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18170.16668837804960837No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17780.1631105867761165No Hit
GGATGAGGCTGACTATTACTGTAATTCCCGGCACAGTAGTGATTATTATC17080.1566889101313875No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16230.14889115991993088No Hit
GTAGTGATTATTATCTGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTG15880.14568032159756636No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15850.14540510688422084No Hit
GTAAAGGGGCCTGTCCTGGCTTCTTTTGGTACCAGGTTGCATAATTGGTT15720.1442125097930569No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15600.14311165093967476No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTGCT15500.14219426856185635No Hit
GTCCGGATCACATGCCAAGGAGACAGCCTCAGAACCAATTATGCAACCTGGTACCAAAAGAAGCCAGGACAGGCC15360.14090993323291054No Hit
GGGACAGACAGTCCGGATCACATGCCAAGGAGACAGCCTCAGAACCAATT15250.13990081261731027No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15100.13852473905058263No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14620.13412130363705418No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14440.13247001535698102No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGA13850.12705745932785228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13720.12586486223668833No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13660.12531443280999727No Hit
GCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAACATGTGTCCCCTGAACTGGAGCCAGAGAATCGGT13430.12320445334101487No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12970.11898449440305012No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12950.11880101792748643No Hit
AGCCAGGACAGGCCCCTTTACTTGTCATTTATGCTAAGGACAAACGGCCC12950.11880101792748643No Hit
GTCCTGGACCTCCTGTGCCAGAACATGAAACATCAGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12860.11797537378744985No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12780.11724146788519511No Hit
AATCACTACTGTGCCGGGAATTACAGTAATAGTCAGCCTCATCCTCCGCC12460.11430584427617613No Hit
GTCTGTGGCCTTGGGACAGACAGTCCGGATCACATGCCAAGGAGACAGCCTCAGAACCAATTATGCAACCTGGTA12430.11403062956283061No Hit
GTCTCTGGTGACTCCCTCACAAATGACTATTGGAGTTGGATCCGGCAGCC12410.11384715308726692No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12390.11366367661170323No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12250.11237934128275744No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCCGGATCAC12190.11182891185606639No Hit
CTATTACTGTAATTCCCGGCACAGTAGTGATTATTATCTGGTGTTCGGCG12120.11118674419159347No Hit
CAATAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGAACTGATTCTTGGTCGTGTCTA12080.11081979124046609No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12040.11045283828933873No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11740.10770069115588345No Hit
CTTCTGAACTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACA11690.10724199996697424No Hit
GGACAAACGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGTTCAGGGGACACATGTTCCTTGACCATCA11640.10678330877806502No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT11390.10448985283351894No Hit
CTACTGTGCCGGGAATTACAGTAATAGTCAGCCTCATCCTCCGCCTGAGC11010.10100379979780891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)