FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975387
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAAGAATAAGAACTACTTAGCTTGGTACCAGCAGAA50780.5206138691616764No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTCTTGGAGCTGTATAAAAGACT49530.5077984430795162No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40590.41614251573990635No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT39080.40066148103265675No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38620.39594540423442176No Hit
ATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAAATTGAGATCAAACGAACTGTGGCTGCACCAT37580.3852829697340645No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACAGAGCCAGGGACTCTGGAGA31090.3187452775154887No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAAGAATAAGAACTA28570.29290937853385374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28170.28880844218756246No Hit
TTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAAATTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT26060.267176002960876No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25370.2601018877635236No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24180.2479016021333071No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23400.23990477625803913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23310.23898206558012358No Hit
CTATAATATTGCTGACAGAAATAAACTGCCGCATCTTCAGCCTGCAGGCTCCTGATGGTGAGAGTGAATTCTGTC22790.23365084832994493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22640.23211299720008574No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21960.22514140541139055No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21560.2210404690650993No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20820.21345373682446042No Hit
GTCTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA20020.2052518641318779No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20020.2052518641318779No Hit
GTACTATAATATTGCTGACAGAAATAAACTGCCGCATCTTCAGCCTGCAGGCTCCTGATGGTGAGAGTGAATT19710.20207363846350218No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19470.19961307665572742No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19120.19602475735272257No Hit
GAATTCACTCTCACCATCAGGAGCCTGCAGGCTGAAGATGCGGCAGTTTATTTCTGTCAGCAATATTATAGTACT18420.18884811874671284No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17840.1829017610445905No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17120.17552007562126623No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16910.1733670840394633No Hit
CTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGC16670.17090652223168853No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT16510.16926614769317205No Hit
GTGCAGCCACAGTTCGTTTGATCTCAATTTTGGTCCCTCCGCCGAAAGCC15350.15737343228892736No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15300.15686081524564097No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCAC15190.15573305775041085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15160.15542548752443902No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCTACCCGGGAA15100.15481034707249533No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14490.14855641914440115No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14150.14507062325005357No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACGCAGTCTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGG13710.14055959326913317No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13080.13410061852372443No Hit
GTTTATTTCTGTCAGCAATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAAATTGAGATCAAACGAA12760.1308198694466914No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12720.13040977581206228No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCACCATCAGGAGCC12470.12784669059563025No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12340.12651388628308557No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12270.12579622242248462No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12240.12548865219651278No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA12190.12497603515322635No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG11870.12169528607619334No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11850.12149023925887878No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11790.12087509880693509No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11790.12087509880693509No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11750.12046500517230596No Hit
GTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA11570.11861958381647489No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11570.11861958381647489No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11450.11738930291258752No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTCTTGGAGCTG11390.11677416246064383No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11380.11667163905198655No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11280.11564640496541372No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11190.11472369428749819No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAATTTTGGTCC11070.11349341338361082No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC10830.11103285157583606No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG10800.11072528134986422No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10750.1102126643065778No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10670.10939247703731954No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10660.10928995362866226No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10660.10928995362866226No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT10560.10826471954208945No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10550.10816219613343217No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10500.10764957909014576No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10440.10703443863820207No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10390.10652182159491565No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10300.10559911091700012No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10260.105189017282371No Hit
GGATAACAGACTACGCGGCACCCGTGAAAGGCAGATTCACCTTCTCAAGAGATGAATCAGAAAACACGGTATTT10190.10447135342177002No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10080.10334359592653992No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10020.10272845547459623No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9920.10170322138802343No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9890.10139565116205158No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9890.10139565116205158No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9880.1012931277533943No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9870.10119060434473702No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9850.10098555752742246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)