FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975387
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTCTTGGAGCTGTATAAAAGACT47380.4857559102182006No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42150.43213616749044226No Hit
CTTTTATACAGCTCCAAGAATAAGAACTACTTAGCTTGGTACCAGCAGAA41260.4230115841199442No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39020.4000463405807131No Hit
ATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAAATTGAGATCAAACGAACTGTGGCTGCACCAT36810.3773886672674538No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT34970.358524360074514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32240.3305354695110761No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACAGAGCCAGGGACTCTGGAGA27510.2820418972161819No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27030.27712077360063236No Hit
CTATAATATTGCTGACAGAAATAAACTGCCGCATCTTCAGCCTGCAGGCTCCTGATGGTGAGAGTGAATTCTGTC26990.27671067996600324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25510.2615372154847256No Hit
TTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAAATTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT23840.24441580623895953No Hit
GTACTATAATATTGCTGACAGAAATAAACTGCCGCATCTTCAGCCTGCAGGCTCCTGATGGTGAGAGTGAATT23010.23590636332040515No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAAGAATAAGAACTA22870.23447103559920318No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22010.22565402245467697No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22000.22555149904601968No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20590.21109569842534298No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20240.20750737912233813No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19660.20156102142021579No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18820.1929490550930041No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18780.19253896145837499No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17960.1841320419484779No Hit
GTCTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA17770.18218409718398954No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17720.18167148014070314No Hit
GAATTCACTCTCACCATCAGGAGCCTGCAGGCTGAAGATGCGGCAGTTTATTTCTGTCAGCAATATTATAGTACT17570.1801336290108439No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17250.17685287993381088No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16540.16957371791914388No Hit
GTGCAGCCACAGTTCGTTTGATCTCAATTTTGGTCCCTCCGCCGAAAGCC16430.16844596042391377No Hit
GAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16340.16752324974599825No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16340.16752324974599825No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16250.16660053906808273No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16240.16649801565942543No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16090.16496016452956622No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14610.1497867000482885No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCAC14250.1460958573366264No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT13600.13943183577390308No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13490.13830407827867297No Hit
CTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGC13100.134305665341039No Hit
GTTTATTTCTGTCAGCAATATTATAGTACTCCTCCGGCTTTCGGCGGAGGGACCAAAATTGAGATCAAACGAA12930.13256276739386522No Hit
GAAATAAACTGCCGCATCTTCAGCCTGCAGGCTCCTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCA12900.13225519716789336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12820.1314350098986351No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG12560.1287694012735458No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACGCAGTCTCCAGAGTCCCTGGCTCTGTCTCTGGGCGAGAGGG12420.12733407355234383No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12400.12712902673502927No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCTACCCGGGAA12250.12559117560517005No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12000.12302809038873802No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11800.12097762221559237No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11750.12046500517230596No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11720.12015743494633413No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11570.11861958381647489No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11530.11820949018184576No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11480.11769687313855937No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11420.11708173268661567No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10930.11205808566240887No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10870.11144294521046517No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10830.11103285157583606No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10560.10826471954208945No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG10550.10816219613343217No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCACCATCAGGAGCC10530.1079571493161176No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10510.10775210249880304No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTCTTGGAGCTG10410.10672686841223021No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10340.10600920455162924No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10260.105189017282371No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10200.1045738768304273No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10150.10406125978714091No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT10140.10395873637848363No Hit
TCTTCAGCCTGCAGGCTCCTGATGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10090.1034461193351972No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC9990.10242088524862439No Hit
GTCCCTGGCTCTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA9840.10088303411876517No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9800.10047294048413603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)