FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653589
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21460.3283408992501404No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT19640.30049465336778924No Hit
GTGTTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC17640.26989438316740333No Hit
CCCCAACACTTAGGCCGGAATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC15720.2405181237750329No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15600.2386821075630098No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAACACTTAGGCCGGAATCCCATGTT15590.23852910621200785No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14790.22628899813185352No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13990.21404889005169916No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAACACTTAGG13860.21205987248867408No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13330.2039508008855718No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13290.20333879548156408No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12730.19477071982545607No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11480.1756455509502149No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11380.1741155374401956No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10470.16019241449902002No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10310.15774439288298914No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10240.15667338342597567No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9850.1507063307369004No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGAAA9740.1490233158758792No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9620.14718729966385602No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9110.13938423076275763No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8740.13372318077568623No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8730.1335701794246843No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8710.13326417672268046No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8580.13127515915965537No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGC8540.13066315375564766No Hit
GTACCAACAGTTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACGATAATAAGCGACCCTCAGGGATTCCTGA8250.12622611457659172No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8240.12607311322558978No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8180.1251551051195782No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8140.12454309971557048No Hit
GATTATTACTGCGGAACATGGGATTCCGGCCTAAGTGTTGGGGTGTTCGG8100.12393109431156277No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8100.12393109431156277No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7340.11230299163541614No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7210.11031397407239105No Hit
CCCTCAGTGTCTGCGGCCTCAGGACAGAAGGTCACCATCTCCTGCTCTGG7140.10924296461537755No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGC7090.1084779578603679No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7050.10786595245636019No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6900.10557093219133126No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6890.10541793084032933No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6790.10388791733031003No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAACACTTAGGC6700.10251090517129266No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6690.10235790382029074No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6610.1011338930122753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3700.089.05961670
ATGTCGT700.058.7901737
ACGTCCG250.00778490651.01485469
GTATGTC200.00789266350.839218
TCGTATG4000.049.150940
CGTATGC4050.048.6501741
GTCGTAT850.048.5846939
TGTCGTA850.048.50555838
TATACAC4300.048.0768173
CTTATAC4300.046.5147631
AAAGGGG4300.046.10334468
TTATACA4350.045.9660382
GAGCGAT4250.045.71858632
CCGTCTT4000.045.40150547
ATGCCGT4250.044.91983844
GCCGTCT4050.044.74926846
CGAGACA4400.044.6905623
GTATGCC4500.044.59507442
ACGAGAC4450.044.17827222
ATCTCGT3450.043.7376137
Produced by FastQC (version 0.11.5)