FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653589
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39800.6089453769876788No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21970.33614396815123876No Hit
GTGTTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC17320.2649983399353416No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15060.2304200346089056No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14790.22628899813185352No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCTCAGGACAGAAGGTCACCATCTCCTG13660.2089998454686355No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAACACTTAGGCCGGAATCCCATGTT13510.20670482520360653No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13290.20333879548156408No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13260.2028797914285583No Hit
CCCCAACACTTAGGCCGGAATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC12730.19477071982545607No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12450.19048668199740204No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11830.18100059823528242No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCTCAGGACAGAAGGTCAC11670.17855257661925156No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11340.17350353203618787No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10970.1678424820491165No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAACACTTAGG10030.15346035505493513No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9510.1455042848028348No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9470.1448922793988271No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGAAA9350.14305626318680395No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9110.13938423076275763No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9070.13877222535874992No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8790.13448818753069589No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC8670.13265217131867274No Hit
GATTATTACTGCGGAACATGGGATTCCGGCCTAAGTGTTGGGGTGTTCGG8510.13020414970264188No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8300.12699112133160137No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8280.1266851186295975No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8030.12286008485454926No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7930.12133007134452996No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7710.11796404162248754No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7700.1178110402714856No Hit
GTACCAACAGTTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACGATAATAAGCGACCCTCAGGGATTCCTGA7270.11123198217840263No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7130.10908996326437562No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGC7120.10893696191337368No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7050.10786595245636019No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGC7050.10786595245636019No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG7010.10725394705235249No Hit
CCCTCAGTGTCTGCGGCCTCAGGACAGAAGGTCACCATCTCCTGCTCTGG6940.10618293759533898No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6860.10495892678732353No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6800.10404091868131196No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6550.10021588490626372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGAT150.0024185368.538936
GAATACG1150.041.192619
AATACGT1100.039.9889310
GTATCAA19650.039.2850531
GTGGTAT2250.039.124241
GCTAGTT350.001476699338.7002321
ATACGTA1150.038.25028211
GGTATCA8450.037.26331
ATGGCAA2150.036.8878470
GACTGAG2750.036.04948470
ACGTACC1250.035.19295513
TACGTAC1250.035.1875712
GTGCTAG1550.034.949691
CCGTTAA502.0815361E-433.8471516
GGTACCG502.0843456E-433.839386
TATCAAC23200.032.9642262
ATCAACG23500.032.538423
TCAACGC23700.032.121084
CAACGCA23950.031.9319385
AACGCAG24350.031.8243036
Produced by FastQC (version 0.11.5)