FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976125
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37820.38745037776924063No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGAGGTACTACCTACATATGAGCAG31860.32639262389550516No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29260.2997566909975669No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGAGGTACTACCTACATATGAGCAGCAGTAATAAT25810.264412856959918No Hit
TATGTAGGTAGTACCTCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC25590.2621590472531694No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24860.2546804968625944No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24600.2520169035728006No Hit
GATTATTACTGCTGCTCATATGTAGGTAGTACCTCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT23300.23869893712383147No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22790.2334741964400051No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21930.22466384940453324No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21470.21995133819951335No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCA21410.2193366628249456No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21380.21902932513766168No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21300.21820975797157124No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20400.20898962735305415No Hit
GTGTTGGTACCAGGAGACAAGGTTATAAGTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA20130.20622358816749903No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19720.2020233064412857No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT19660.2014086310667179No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19540.20017928031758228No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19420.19894992956844665No Hit
ACCTACATATGAGCAGCAGTAATAATCAGACTCGTCCTCAGCCTGGAGCC19380.19854014598540146No Hit
GTAATAATCAGACTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG18290.18737354334741965No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17760.18194391087207068No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17390.17815341272890253No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17360.17784607504161865No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17120.17538737354334744No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTTGTCTCCTGGTACCAACACCA17030.17446536048149572No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16760.17169932129594057No Hit
CTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA16730.17139198360865668No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16600.17006018696375974No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16380.16780637725701114No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16120.16514278396721732No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC16110.16504033807145602No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15220.1559226533487002No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14940.1530541682673838No Hit
GGTACTACCTACATATGAGCAGCAGTAATAATCAGACTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGC14830.15192726341400947No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14720.15080035856063514No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14590.14946856191573824No Hit
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA14410.14762453579203483No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAC14250.145985401459854No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14020.1436291458573441No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13740.14076066077602767No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13670.14004353950569856No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13510.13840440517351774No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13340.1366628249455756No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13250.13574081188372392No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13150.1347163529261109No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12520.12826226149314893No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12300.1260084517864003No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12270.1257011140991164No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGAGGTACTAC12170.12467665514150339No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAGAGGTA12030.12324241260084517No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA11840.12129594058138046No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11830.12119349468561916No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11610.11893968497887053No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11360.116378537584838No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA11310.1158663081060315No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11190.1146369573568959No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11130.11402228198232808No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11130.11402228198232808No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT10920.11187091817134076No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC10900.11166602637981816No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10900.11166602637981816No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG10830.11094890510948904No Hit
GGTTATAAGTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATC10760.11023178383915994No Hit
GTATTACTGTGCAAAAGATGGATATTGTAGTAGTAGTAGCTGCTTCTACA10750.11012933794339863No Hit
GGTAGTACCTCTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGG10350.10603150211294661No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10270.1052119349468562No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10220.10469970546804969No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10110.10357280061467537No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10100.10347035471891407No Hit
GCTTGGTCCCTCCGCCGAAAACCACAGAGGTACTACCTACATATGAGCAGCAGTAATAATCAGACTCGTCCTCAG9970.10213855807401716No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9950.10193366628249456No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC9940.10183122038673324No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC9940.10183122038673324No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT9880.10121654501216545No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTACTGTGCAAAAGATGGATAT9880.10121654501216545No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9850.10090920732488155No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9800.10039697784607504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA100.009560199131.2000370
AGGGGGG2200.062.6181970
GGTATCA7850.050.07741
CGTATGC1900.043.63952641
CTACGCT2500.042.42421328
ACTACGC2500.042.40898527
TCGTATG1800.042.18595540
TATACAC2650.041.263113
TCTCGTA1800.040.17694538
GCGTATG350.001344685239.4466140
GTATCAA20550.039.423041
ATCGCGT350.001365332639.32452837
GACTACG2700.039.2595326
AAACGCA350.001383779739.21731633
TCCATAC350.001412709439.0525863
TACGCTG2750.038.58725429
AGACTAC2750.038.5397925
CGAGACT2800.037.83800523
ACGCTGC2900.037.7814230
TACACAT3200.037.3745425
Produced by FastQC (version 0.11.5)