FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779091
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38300.49159854240390405No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTGTCCGGGAATCTGGGGTCCCT38150.4896732217417477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37770.48479574273095183No Hit
GTTTTACACAGCTCAGATAATAAGAACTACTTAGGTTGGCACCAGCAGAA37010.47504078470936No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTTCTTATTATCTGAGCTGTGTAAAACACT32050.4113768481473923No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26720.342963787285439No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25690.3297432520719659No Hit
GTGTAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGACTCTGGAGA24530.31485410561795735No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23790.30535585701798634No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23400.30035002329638005No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22590.2899532917207361No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22490.2886697446126319No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21800.279813269566713No Hit
GTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21110.2709567945207941No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21020.26980160212350035No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20350.2612018364992023No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20240.25978993468028766No Hit
GTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA19880.2551691650911126No Hit
ATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA19190.24631269004519368No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18260.23437570193982474No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16990.21807465366690154No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16880.21666275184798697No Hit
GTCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAACAAC16560.21255540110205356No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16120.20690779382639513No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15890.2039556354777555No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15720.20177360539397837No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15570.1998482847318221No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15000.1925320662156282No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT14780.189708262577799No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13600.17456240670216958No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13580.17430569728054873No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTGTCCGGGAA12980.16660441463192363No Hit
CTACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTGTCCGGGAAT12760.1637806109940944No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12690.16288212801842147No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12630.16211199975355894No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT12520.16070009793464435No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGTTGTTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA12250.15723452074276303No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12020.1542823623941234No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11840.15197197759953587No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11710.15030336635900043No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAACAACC11520.14786462685360247No Hit
GCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11370.14593930619144618No Hit
GTTCAGAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGTTGTTGATGGTGAGAGTGAAAT11340.14555424205901493No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11320.1452975326373941No Hit
GTGCTGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGC11300.14504082321577325No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11200.14375727610766906No Hit
GATTTCACTCTCACCATCAACAACCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCT11150.14311550255361696No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10840.139136506518494No Hit
GCTCAGATAATAAGAACTACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGG10750.13798131412120024No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAG10710.13746789527795855No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10540.13528586519418143No Hit
TTCCCGGACAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTT10520.13502915577256058No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10510.13490080106175018No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10500.13477244635093977No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10450.13413067279688767No Hit
ACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATT10440.13400231808607724No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10440.13400231808607724No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10310.1323337068455418No Hit
GCTCATTTACTGGGCATCTGTCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTT10270.1318202880023001No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10200.13092180502662717No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACAGCTCAGATAATAAGAACTA10070.12925319378609174No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10040.12886812965366048No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9990.1282263560996084No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC9810.12591597130502086No Hit
GTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTTCTTATTATCTGAGCTG9800.12578761659421042No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9710.12463242419691666No Hit
GTCTGAGACAGATTTCACTCTCACCATCAACAACCTGCAGGCTGAGGATG9700.12450406948610623No Hit
GATAATAAGAACTACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCAT9690.12437571477529581No Hit
CCTAAGTAGTTCTTATTATCTGAGCTGTGTAAAACACTCTGGCTGGACTT9680.12424736006448539No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9620.1234772317996229No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9540.12245039411313956No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGG9470.12155191113746662No Hit
GTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACA9360.12014000931855201No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9170.11770126981315406No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9150.11744456039153321No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8980.11526253030775609No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8970.11513417559694566No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8960.11500582088613526No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8950.11487746617532484No Hit
GGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGAT8790.11282379080235813No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8740.11218201724830604No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8720.1119253078266852No Hit
CCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGAC8670.1112835342726331No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8620.110641760718581No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8610.11051340600777058No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8590.11025669658614974No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8570.10999998716452891No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8500.109101504188856No Hit
CCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACAG8430.10820302121318306No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8280.10627770055102678No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8270.10614934584021636No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTGCCAACCTA8200.10525086286454342No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8140.10448073459968091No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8100.10396731575643924No Hit
TTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGA8060.10345389691319756No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8000.10268376864833505No Hit
GACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAA7850.10075844798617876No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7820.1003733838537475No Hit
CACCATCAACAACCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGC7800.10011667443212667No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGTTGTTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA7800.10011667443212667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAA300.002998265264.9182770
AGGCTCG401.1505363E-464.9182770
TCGTATG1950.056.61956440
CTTATAC2200.052.380461
CGTATGC2150.051.45017241
GAGCGAT2200.051.12219232
ACGAGAC2350.050.48253622
ATGCCGT2250.049.4257144
TACACAT2350.047.567275
CGAGACA2500.047.45967523
TGAGCGA2600.045.84632531
TGTCGTA605.811671E-945.81471338
CCGTCTT2450.045.64341747
CCCACGA2600.045.61379219
ATCTCCG2600.045.5991610
TCTCCGA2600.045.5991611
GGTATCA7150.045.5070081
TTTGTAC306.9063273E-445.164884
CACGAGA2700.043.9328621
ACACATC2650.043.460556
Produced by FastQC (version 0.11.5)