FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779091
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39350.505075787038998No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTGTCCGGGAATCTGGGGTCCCT32680.4194631949284487No Hit
GTTTTACACAGCTCAGATAATAAGAACTACTTAGGTTGGCACCAGCAGAA31460.4038039202095776No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30620.3930221245015024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29390.3772344950718209No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTTCTTATTATCTGAGCTGTGTAAAACACT29190.3746674008556125No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25820.3314118633125014No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25010.3210151317368574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22120.2839206203126464No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22040.28289378262616305No Hit
GTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA20360.2613301912100127No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20340.26107348178839185No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19910.25555422922354387No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19810.2542706821154397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19420.2492648483938333No Hit
ATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA19170.24605598062357287No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18470.23707115086684352No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18100.23232202656685805No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGTTGTTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA17300.2220536497020245No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17090.21935820077500576No Hit
GTGTAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGACTCTGGAGA16390.2103733710182764No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16320.2094748880426035No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15850.20344221663451384No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15670.20113183183992628No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15570.1998482847318221No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT15390.19753789993723456No Hit
GTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA15120.19407232274535324No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14940.1917619379507657No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14660.18816800604807396No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14620.1876545872048323No Hit
GTTCAGAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGTTGTTGATGGTGAGAGTGAAAT14060.18046672339944886No Hit
GTCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAACAAC13990.17956824042377592No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13900.17841304802648214No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12610.16185529033193813No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12510.16057174322383394No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12320.15813300371843597No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTGTCCGGGAA11910.1528704605752088No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11600.1488914645400858No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11390.14619601561306703No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11220.1440139855292899No Hit
TCCTCAGCCTGCAGGTTGTTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACC11190.14362892139685865No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11160.1432438572644274No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11060.1419603101563232No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAG10960.140676763048219No Hit
ATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCACCATCAACAACC10710.13746789527795855No Hit
GATTTCACTCTCACCATCAACAACCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCT10630.1364410575914752No Hit
GAGTGAAATCTGTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCA10490.13464409164012933No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10420.1337456086644564No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10420.1337456086644564No Hit
GCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10290.13207699742392096No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10240.13143522386986886No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10220.13117851444824802No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10110.12976661262933342No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGTTGTTGATGGTGAGAGTGAAATCTGTCTCAGACCCGCTGCCA10050.1289964843644709No Hit
GTCTGAGACAGATTTCACTCTCACCATCAACAACCTGCAGGCTGAGGATG10020.12861142023203964No Hit
TTCCCGGACAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTT9990.1282263560996084No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9750.12514584304015833No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9720.12476077890772708No Hit
GTGCTGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGC9670.12411900535367498No Hit
CTACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTGTCCGGGAAT9490.12180862055908746No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9330.11975494518612076No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGG9320.11962659047531034No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9230.11847139807801656No Hit
GTCTCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCAGATTCCCGGAC9160.11757291510234363No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9140.11731620568072279No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9070.11641772270504985No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9070.11641772270504985No Hit
GCTCATTTACTGGGCATCTGTCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTT8990.11539088501856651No Hit
ACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATT8930.11462075675370399No Hit
GGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGAT8840.11346556435641023No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8780.11269543609154771No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8780.11269543609154771No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8750.11231037195911645No Hit
CCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTCTGAACCTCCTCGAACTTTTGGCCAGGGGAC8700.11166859840506435No Hit
GTCCTGGTTTCTGCTGGTGCCAACCTAAGTAGTTCTTATTATCTGAGCTG8690.11154024369425394No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8560.10987163245371849No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8560.10987163245371849No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8490.10897314947804558No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8480.10884479476723516No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8430.10820302121318306No Hit
GCTCAGATAATAAGAACTACTTAGGTTGGCACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGG8350.10717618352669972No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACAGCTCAGATAATAAGAACTA8340.1070478288158893No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8310.10666276468345803No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGAGACAGATTTCACTCTCAC8150.10460908931049133No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8100.10396731575643924No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8090.10383896104562881No Hit
GAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGTTGTT8060.10345389691319756No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8030.10306883278076631No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8000.10268376864833505No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7990.10255541393752463No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7980.1024270592267142No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7830.10050173856455792No Hit
GGCCAAAAGTTCGAGGAGGTTCAGAATATTGCTGACAGTAATAAACTGCC7810.10024502914293709No Hit
GTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACA7810.10024502914293709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATT100.009004829133.8499170
CTACATG300.0026573966.9249670
CGGTATT200.00674220852.9066251
TATCACG200.00797551650.7059972
GCGACCA400.00829909250.19371870
GGTATCA7700.045.2735521
GTATTGG306.934901E-445.1270371
GTATCAA20250.038.608691
GTGGTAT2050.034.6707731
TATCAAC23000.033.8039972
ATCAACG24000.032.1096763
TCAACGC24100.031.9764424
CAACGCA24150.031.9122875
AACGCAG24300.031.7173336
GCTTAGC553.643137E-430.7684331
GGTCTGA18600.030.58398670
AATGGTA2201.4624675E-930.42043370
ACGCAGA25500.030.3573177
GAGTTTA2800.030.2189961
GTTGCAC450.005109389330.0441423
Produced by FastQC (version 0.11.5)