FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865120
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25620.29614388755317184No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22280.25753652672461624No Hit
CTCCTAAGCTGCTCATTTACTGGGGGTCTACCCGGGAATCCGGGGTCCCT21540.2489828000739782No Hit
GTAATACACGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA20270.23430275568707232No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16570.191534122433882No Hit
ATATTATAGCACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATGAGACGAACTGTGGCTGCACCAT16520.19095616793046052No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15580.18009062326613648No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT15380.17777880525245054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15160.17523580543739598No Hit
GTGTATTACTGTGCGAGAGGCATCACGGTTCTCGGAGTGGTTAGGTATCA13850.16009339744775292No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13670.15801276123543556No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13290.15362030700943222No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTAAGTAATTCTTATTGCTTAAACTATATAAAATACT12870.14876548918069168No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGTTGGATCCGGCAGTC12660.14633808026632145No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12150.14044294433142224No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12100.13986498982800072No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12000.13870908082115777No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11390.13165803587941557No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11380.13154244497873127No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10880.12576289994451637No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10770.1244914000369891No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10550.12194840022193452No Hit
TTATAGCACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATGAGACGAACTGTGGCTGCACCATCTGTCTT10250.11848067320140558No Hit
GTCCCTGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCAC10170.11755594599593121No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9920.11466617347882374No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9620.1111984464582948No Hit
ATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCGGCC9540.11027371925282042No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9420.10888662844460884No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTTACCAATATTATAGCACT9400.10865544664324024No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9340.10796190123913446No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGGGTCTACCCGGGAA9240.10680599223229148No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9140.10565008322544849No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9120.10541890142407989No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8880.10264471980765676No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT8870.10252912890697244No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8830.10206676530423525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26600.038.0065421
ATTAGGC558.141498E-637.110423
GCCGTCT2850.036.67526646
CGTATGC2750.036.50209841
GTGGTAT3000.036.308831
TCGTATG2900.035.74668540
ATGCCGT2900.034.76125344
GTACGGT400.002772766634.0395281
TTAGGCT502.0214531E-434.0178874
ATCTCGT2950.033.80274637
CCGTCTT3050.033.17118547
CACGAGA3200.032.96054521
TATCAAC30900.032.8068962
ATCTCCG3150.032.39798710
CGTCTTC3250.032.25494848
GTATGCC3450.032.13414442
TTATACA3400.032.0168342
CCCACGA3350.031.47923919
TATACAC3600.031.1830643
CTACATC3200.031.02957333
Produced by FastQC (version 0.11.5)