FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865120
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31530.364458109857592No Hit
CTCCTAAGCTGCTCATTTACTGGGGGTCTACCCGGGAATCCGGGGTCCCT21120.24412798224523763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20860.24112261882744593No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20590.23800166450896987No Hit
GTAATACACGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA19730.22806084705012022No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17610.203555576105049No Hit
ATATTATAGCACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATGAGACGAACTGTGGCTGCACCAT17170.1984695764749399No Hit
GTGTATTACTGTGCGAGAGGCATCACGGTTCTCGGAGTGGTTAGGTATCA14210.16425466987238765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13810.1596310338450157No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGTTGGATCCGGCAGTC13140.15188644349916774No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTAAGTAATTCTTATTGCTTAAACTATATAAAATACT12360.1428703532457925No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12080.13963380802663214No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11790.1362816719067875No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11730.1355881265026817No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11720.1354725356019974No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11290.1305021268725726No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11120.12853708156093951No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11010.12726558165341223No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10970.12680321805067504No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10330.11940540040687997No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9740.11258553726650637No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9590.1108516737562419No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9580.11073608285555761No Hit
TTATAGCACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATGAGACGAACTGTGGCTGCACCATCTGTCTT9560.11050490105418902No Hit
GTCCCTGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCAC9310.10761512853708155No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGGGTCTACCCGGGAA9230.10669040133160718No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9030.10437858331792123No Hit
ATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCGGCC9000.10403181061586832No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8930.10322267431107823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28800.039.8670231
TTTAGAC451.10375404E-437.704663
GTGGTAT2850.036.949321
TATCAAC34400.033.0521132
ATCAACG35150.032.3412673
AACGCAG35600.031.6538146
TCAACGC35600.031.6464984
CAACGCA35700.031.5614975
ACGCAGA37050.030.3233917
CGGGTAC1351.0913936E-1130.18640917
CGCAGAG37350.030.0798288
AGAGTAC38250.029.2833311
GCAGAGT38700.029.1182389
GCTTATC500.00834119927.1756291
AATAGTC907.1522154E-626.71130836
GTCTACT5600.026.51118767
TGTAAGG659.5229107E-426.1077542
TACGGGT12300.025.94544815
ACGGGTA3800.025.91212716
GCGTAGA13350.025.7432270
Produced by FastQC (version 0.11.5)