FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236517
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG68000.5499317841970632No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCAC56210.4545833174958371No Hit
GTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC55980.45272325410811176No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC50510.4084860944087303No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAG48060.38867237571339497No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47030.38034252662923357No Hit
GTAATAGACAGCCGTGTCCTCGTGTCTCAGGCTGTTCATTTGCAGAAACA45320.36651335970310156No Hit
GACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAG40020.32365102946421276No Hit
GTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC38430.31079233039254617No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC36550.29558833400592144No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC36500.29518397239989425No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG36310.29364739829699066No Hit
GCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGA34260.2770685724498733No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33150.2680917447960683No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33010.266959532299192No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32500.262835043917714No Hit
CACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC31120.25167466359136187No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30870.2496528555612256No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30650.2478736644947057No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30520.24682232431903484No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC29250.23655153952594263No Hit
CAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA28240.22838343508419212No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28220.22822169044178126No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT28050.22684686098128856No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27790.2247441806299469No Hit
ATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACT27160.21964922439400347No Hit
GTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA27040.21867875653953806No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26810.21681869315181274No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26660.21560560833373096No Hit
CCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTC26550.214716012800471No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26390.21342205566118377No Hit
CAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAAT26130.21131937530984207No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG25900.20945931192211673No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG25590.20695226996474775No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24770.2003207396259008No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24750.20015899498348994No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24670.19951201641384633No Hit
GTCTATTACTGTGCGAGAGGTGGTGTAGTAGACTTTGACTACTGGGGCCA24200.19571101731719015No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24180.19554927267477923No Hit
GGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT24020.19425531553549205No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATAC24020.19425531553549205No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23940.19360833696584842No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23900.19328484768102663No Hit
GTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGA22730.18382278609998892No Hit
ATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTTCAAAT22650.18317580753034532No Hit
CTCCAGAGCCCCACTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG22480.18180097806985265No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT22370.1809113825365927No Hit
GATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTA22060.17840434057922375No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGC21640.1750077030885948No Hit
GCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC20960.1695083852466242No Hit
TCTCTGGCCTCCAGTCAGAGGATGAGGCTGACTATTACTGTATGATTTGG20260.16384732276224268No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20100.16255336562295544No Hit
CTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCC20000.16174464241090095No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19980.16158289776849005No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19930.1611785361624628No Hit
ACCATATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTTTCTG19870.1606933022352301No Hit
AGTATATCCTGTACGTACCAACATTGATGCCACTGCGTAGGGTGCAGGTGAGACTGGCTGATGCTCCAGGAGATG19650.15891411116871018No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG19400.1568923031385739No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGC19370.15664968617495756No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGTACG19360.1565688138537521No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19130.15470875046602675No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18810.15212083618745234No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18700.15123124065419238No Hit
GGATGAGGCTGACTATTACTGTATGATTTGGCACAACAACGCTGTGGTATTCGGCGGAGGGACTAAGCTGACCGT18300.14799634780597437No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18190.14710675227271441No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18130.1466215183454817No Hit
GCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG18000.14557017816981083No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT17840.14427622103052362No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTC17770.14371011478208548No Hit
GAATACCACAGCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCAT17370.14047522193386747No Hit
GTGGTATTCGGCGGAGGGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA17190.13901952015216934No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16660.1347332871282805No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16600.1342480532010478No Hit
GTATATGGTACTACTAGCAGTAGTAATGAATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTG16460.13311584070417148No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG16440.1329540960617606No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16310.1319027558860897No Hit
ATGCAGGGATTTTACTCATCTCTGGCCTCCAGTCAGAGGATGAGGCTGAC15860.12826350143184445No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15690.12688867197135179No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15440.12486686394121553No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15360.12421988537157193No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15350.12413901305036647No Hit
CAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCC15290.12365377912313376No Hit
CCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTC15170.12268331126866837No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT15150.12252156662625746No Hit
GTCCTCCCCAGTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCCCAGCCGCTTCT15060.12179371573540841No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG15010.12138935412938116No Hit
GTCTCACCTGCACCCTACGCAGTGGCATCAATGTTGGTACGTACAGGATA14910.12058063091732664No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT14880.1203380139537103No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC14630.11831620592357404No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14480.11710312110549227No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT14410.11653701485705413No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCCTACGCAGTGGCATCAAT14330.11589003628741051No Hit
GTATCAACGCAGAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC14220.11500044075415058No Hit
CTGCTTATCTGAGTCTGATTTGAACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATAT14130.11427258986330152No Hit
GTATCTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAG14030.11346386665124701No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13850.1120081648695489No Hit
GAGTACGGGACTGTGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA13810.1116846755847271No Hit
GCGTTGTTGTGCCAAATCATACAGTAATAGTCAGCCTCATCCTCTGACTGGAGGCCAGAGATGAGTAAAATCC13740.11111856933628894No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13720.11095682469387805No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTG13440.10869239970012544No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13250.10715582559722187No Hit
CTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCT13140.10626623006396191No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13120.10610448542155101No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12990.10505314524588016No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT12970.10489140060346927No Hit
GATTTTACTCATCTCTGGCCTCCAGTCAGAGGATGAGGCTGACTATTACT12840.1038400604277984No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12770.10327395417936025No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12730.10295046489453845No Hit
GAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAGCGGC12570.10165650775525124No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT12530.10133301847042944No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC12460.10076691222199129No Hit
CTCCTGAGGTTCAAATCAGACTCAGATAAGCAGTGGGGCTCTGGAGTCCC12420.10044342293716949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)